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Human Interleukin 8,IL-8 ELISA KIT

  • 中文名： 人白介素8(IL-8/CXCL8)ELISA KIT
货号: IPD-E04641h
Price： ￥2500
数量：
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产品详情

  • 产品描述：人白介素8(IL-8/CXCL8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、唾液、尿液、脑脊液（CSF）、组织匀浆、细胞裂解物样本中的CXCL8含量。CXCL8，又称白细胞介素-8或CXCL8，是一种促炎CXC趋化因子，主要由免疫细胞和肿瘤细胞分泌。它通过两种受体CXCR1和CXCR2作用于人类中性粒细胞，参与炎症反应和肿瘤发生发展。CXCL8的研究机制涉及信号通路、转录因子调控以及与肿瘤细胞、血管生成等生物学过程的关系。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.110 pg/mL。适用于炎症机制研究、药物作用评价、疾病模型构建等科研场景，助力免疫调控网络及细胞信号通路的深入探索，满足体外实验中IL - 8蛋白表达量监测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：(Ala-IL-8)77 ELISA Kit; (Ser-IL-8)72 ELISA Kit; 9E3 ELISA Kit; Beta thromboglobulin like protein ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; chemokine; CXC motif; ligand 8 ELISA Kit; CXCL8 ELISA Kit; Emoctakin ELISA Kit; GCP-1 ELISA Kit; GCP/IL-8 protein I ELISA Kit; GCP/IL-8 protein II ELISA Kit; GCP/IL-8 protein III ELISA Kit; GCP/IL-8 protein IV ELISA Kit; GCP/IL-8 protein V ELISA Kit; GCP/IL-8 protein VI ELISA Kit; GCP1 ELISA Kit; Granulocyte chemotactic protein 1 ELISA Kit; IL-8 ELISA Kit; IL-8(1-77) ELISA Kit; IL-8(9-77) ELISA Kit; IL8 ELISA Kit; IL8/NAP1 form I ELISA Kit; IL8/NAP1 form II ELISA Kit; IL8/NAP1 form III ELISA Kit; IL8/NAP1 form IV ELISA Kit; IL8/NAP1 form V ELISA Kit; IL8/NAP1 form VI ELISA Kit; IL8_HUMAN ELISA Kit; Inteleukin 8 ELISA Kit; LECT ELISA Kit; LUCT ELISA Kit; Lymphocyte-derived neutrophil-activating factor ELISA Kit; LYNAP ELISA Kit; MDNCF ELISA Kit; MDNCF-b ELISA Kit; MDNCF-c ELISA Kit; MONAP ELISA Kit; Monocyte derived neutrophil activating peptide ELISA Kit; Monocyte derived neutrophil chemotactic factor ELISA Kit; Monocyte-derived neutrophil chemotactic factor ELISA Kit; Monocyte-derived neutrophil-activating peptide ELISA Kit; NAF ELISA Kit; NAP 1 ELISA Kit; NAP-1 ELISA Kit; NAP1 ELISA Kit; Neutrophil activating peptide 1 ELISA Kit; Neutrophil activating protein 1 ELISA Kit; Neutrophil-activating factor ELISA Kit; Neutrophil-activating protein 1 ELISA Kit; Protein 3 10C ELISA Kit; Protein 3-10C ELISA Kit; SCYB 8 ELISA Kit; SCYB8 ELISA Kit; Small inducible cytokine subfamily B member 8 ELISA Kit; T cell chemotactic factor ELISA Kit; T-cell chemotactic factor ELISA Kit; TSG 1 ELISA Kit; TSG1 ELISA Kit

  • 缩写：CXCL8

  • Uniprot No.：P10145

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates

  • 检测范围：31.25 pg/mL-2000 pg/mL

  • 灵敏度：7.110 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Immunology

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

     Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    标准曲线：

  • These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-8 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labled IL-8 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator that can provide stable incubation conditions up to 37°C±5°C.

      • Squirt bottle, manifold dispenser, or automated microplate washer.

      • Absorbent paper for blotting the microtiter plate.

      • 100ml and 500ml graduated cylinders.

      • Deionized or distilled water.


      • 货期：3-5 working days


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