纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MPZL1 |
Uniprot No | O95297 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 38-162aa |
氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSLEVYTPK EIFVANGTQG KLTCKFKSTS TTGGLTSVSW SFQPEGADTT VSFFHYSQGQ VYLGNYPPFK DRISWAGDLD KKDASINIEN MQFIHNGTYI CDVKNPPDIV VQPGHIRLYV VEKENLPV |
预测分子量 | 16 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MPZL1重组蛋白的3篇参考文献示例(注:以下文献信息为模拟内容,实际引用请核实具体论文):
---
1. **文献名称**:*Recombinant Expression and Functional Characterization of MPZL1 in Cell Adhesion*
**作者**:Zhang Y, et al. (2020)
**摘要**:本研究在大肠杆菌系统中成功表达并纯化了重组MPZL1蛋白,验证了其通过胞外结构域介导的细胞间黏附功能,为研究MPZL1在组织发育中的作用提供了工具。
2. **文献名称**:*Structural Insights into MPZL1 Revealed by X-ray Crystallography*
**作者**:Li H, et al. (2019)
**摘要**:通过X射线晶体学解析了重组MPZL1蛋白的胞外区三维结构,揭示了其与配体结合的潜在位点,为靶向MPZL1的药物设计提供了结构基础。
3. **文献名称**:*MPZL1 Regulates EGFR Signaling in Breast Cancer via Recombinant Protein Interaction Assays*
**作者**:Wang X, et al. (2021)
**摘要**:利用重组MPZL1蛋白进行体外互作实验,发现其直接结合EGFR并抑制下游MAPK信号通路,提示MPZL1可能作为乳腺癌治疗的潜在靶点。
---
如需具体文献,建议在PubMed或Web of Science中检索关键词“MPZL1 recombinant”或结合研究领域进一步筛选。
MPZL1 (Myelin Protein Zero Like 1), also known as PZR, is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. Structurally, it features an extracellular Ig-like domain, a single-pass transmembrane region, and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Initially identified for its homology to myelin protein zero (MPZ), MPZL1 is widely expressed in various tissues, including the liver, immune cells, and epithelial tissues, and plays roles in cell adhesion, signal transduction, and immune regulation.
Functionally, MPZL1 interacts with ligands such as CD44 and the tyrosine phosphatase SHP2. mediating cell-cell or cell-matrix communication. It regulates cellular processes like proliferation, migration, and differentiation, with implications in cancer progression, immune response modulation, and tissue homeostasis. Dysregulation of MPZL1 has been linked to pathologies, including hepatocellular carcinoma, breast cancer, and hearing loss. Notably, mutations in MPZL1 are associated with autosomal recessive deafness (DFNB113), highlighting its critical role in inner ear development.
Recombinant MPZL1 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), retains functional domains for structural and mechanistic studies. It is utilized to investigate ligand-receptor interactions, signaling pathways (e.g., SHP2-mediated cascades), and therapeutic targeting. In cancer research, recombinant MPZL1 aids in exploring its dual role as a tumor suppressor or promoter, depending on cellular context. Additionally, it serves as an antigen for antibody development in diagnostic or therapeutic applications.
Despite progress, MPZL1’s full interactome and context-dependent signaling remain understudied. Recombinant protein tools continue to advance our understanding of its biological and pathological significance, offering potential for novel therapeutic strategies.
×