| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPD |
| Uniprot No | A0A0H2ZQ76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-392aa |
| 氨基酸序列 | MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNLTAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQSLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKAISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLLEQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKVRPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIACWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPD(表面活性蛋白D，Surfactant Protein D)重组蛋白的3篇示例参考文献及摘要概括：
1. **文献名称**：*Structural and functional characterization of recombinant human surfactant protein D*
**作者**：Uhlson, C., et al.
**摘要**：本研究通过哺乳动物表达系统成功获得重组人源SP-D蛋白，并利用X射线晶体学解析其三维结构。实验证实重组SP-D保留了天然蛋白的糖识别域活性，可在体外模型中有效凝集流感病毒，证实其先天免疫调节功能。
2. **文献名称**：*Recombinant SP-D modulates TLR4 signaling in airway epithelial cells*
**作者**：Crouch, E.C., Hartshorn, K.L.
**摘要**：文章探讨重组SP-D蛋白通过结合脂多糖(LPS)调控TLR4信号通路的机制。体外实验表明，重组SP-D可抑制LPS诱导的NF-κB活化，降低IL-6等促炎因子表达，提示其在肺部炎症性疾病中的潜在治疗价值。
3. **文献名称**：*Therapeutic efficacy of aerosolized recombinant SP-D in murine pneumonia models*
**作者**：Strong, P., et al.
**摘要**：通过铜绿假单胞菌感染的小鼠模型，验证雾化吸入重组SP-D的治疗效果。实验组动物肺组织细菌载量下降60%，炎性浸润显著减少，证实重组蛋白通过增强肺泡巨噬细胞吞噬功能发挥保护作用。
注：以上文献为示例性内容，实际文献需通过PubMed、Web of Science等学术平台检索验证。建议结合关键词"recombinant surfactant protein D"+"expression"/"therapeutic"等筛选近年高质量研究。
Surfactant Protein D (SPD), also known as SFTPD or collectin-7. is a multifunctional innate immune molecule primarily produced in pulmonary and mucosal tissues. As a member of the collectin family, it plays a critical role in host defense by recognizing pathogen-associated molecular patterns (PAMPs) through its carbohydrate recognition domain (CRD). Structurally, SPD forms trimers that further assemble into octadecamers, creating a spider-like configuration optimized for pathogen opsonization and immune modulation.
Recombinant SPD (rSPD) is engineered using expression systems like mammalian cells (e.g., CHO cells) or insect cells to preserve its post-translational modifications and functional tertiary structure. This technology enables large-scale production of SPD for research and therapeutic applications while overcoming limitations of natural SPD isolation from biological fluids, which yields low quantities and risks contamination.
Current research focuses on rSPD's dual role in inflammation regulation: enhancing pathogen clearance while preventing excessive immune responses. It shows promise in managing respiratory diseases (COPD, COVID-19), allergic asthma, and autoimmune disorders by modulating macrophage polarization and cytokine production. Notably, rSPD's ability to bind SARS-CoV-2 spike protein has sparked interest in COVID-19 therapeutic development. Challenges remain in optimizing its stability and delivery methods for clinical use. As a biomarker, rSPD assists in monitoring disease progression in pulmonary conditions, highlighting its translational potential across diagnostics and therapeutics.
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