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Rabbit Polyclonal H3-5 Antibody

  • 中文名: H3-5抗体
  • 别    名: H3.5; H3F3C
货号: IPDX07340
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/200-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/1000-1/2000 Human,Mouse,Rat

产品详情

AliasesH3.5; H3F3C
WB Predicted band size15 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human H3-5
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于H3-5抗体的假设性参考文献示例(注:H3-5抗体相关文献较少,以下内容为模拟概括,非真实文献):

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1. **文献名称**: "Histone H3.5: A Novel Epigenetic Marker in Primate Spermatogenesis"

**作者**: Tanaka, S. et al.

**摘要**: 本研究报道了一种特异性识别组蛋白变体H3.5的抗体开发,并揭示了H3.5在人类和灵长类动物精子发生中的独特表达模式,提示其在染色质重塑和减数分裂中的关键作用。

2. **文献名称**: "H3K5 Methylation Dynamics in DNA Damage Repair"

**作者**: Lee, J. & Patel, R.

**摘要**: 通过使用H3K5me2特异性抗体,研究者发现该修饰在DNA双链断裂修复过程中被ATM激酶调控,并与同源重组修复通路蛋白(如BRCA1)存在共定位。

3. **文献名称**: "Development of a Monoclonal Antibody Targeting H3K5ac for Cancer Epigenetics Studies"

**作者**: Zhang, Y. et al.

**摘要**: 文章描述了一种高灵敏度抗H3K5乙酰化抗体的制备与验证,并应用于分析多种癌症细胞系中H3K5ac的动态变化,发现其与癌基因激活和不良预后相关。

4. **文献名称**: "H3.5 Variant Regulates Pluripotency in Human Embryonic Stem Cells"

**作者**: Müller, C. et al.

**摘要**: 利用H3.5特异性抗体,研究揭示了该组蛋白变体在维持人胚胎干细胞多能性中的功能,其缺失导致分化相关基因异常激活。

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如需真实文献,建议通过PubMed或Google Scholar检索关键词(如“H3.5 antibody”“H3K5 methylation”),并筛选近年的功能或方法学研究。

背景信息

The H3-5 antibody specifically targets histone H3. a core component of nucleosomes that play a critical role in chromatin structure and gene regulation. Histone H3 undergoes post-translational modifications (e.g., methylation, acetylation) that influence epigenetic mechanisms, including transcriptional activation or repression. The H3-5 variant, part of the H3.3 subfamily, is encoded by the H3F3B gene and is replication-independent, often incorporated into chromatin during DNA repair or transcriptional activity. This antibody is particularly useful in studying epigenetic marks (e.g., H3K4me3. H3K27ac) associated with active promoters or enhancers. It has applications in ChIP-seq, immunofluorescence, and Western blotting to investigate chromatin dynamics in development, differentiation, and diseases like cancer. Research using H3-5 antibodies has advanced understanding of how histone modifications regulate cellular identity, stem cell plasticity, and oncogenic pathways. Its specificity for H3.3 variants also aids in distinguishing replication-dependent and -independent histone deposition mechanisms.

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