| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | p48; IRF-9; ISGF3; ISGF3G |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human IRF9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IRF9抗体的3篇参考文献的简要概括(注：文献信息为示例性虚构，仅供参考)：
1. **"IRF9 Mediates Antiviral Response by Regulating ISGF3 Complex Formation"**
- **作者**: Smith A, et al.
- **摘要**: 本研究利用IRF9特异性抗体，通过免疫共沉淀和Western blot技术，揭示了IRF9在干扰素信号通路中与STAT1/STAT2形成ISGF3复合体的关键作用，证实其在抗病毒基因激活中的必要性。
2. **"Tissue-Specific Expression of IRF9 in Autoimmune Disorders"**
- **作者**: Chen L, et al.
- **摘要**: 通过免疫组化和小鼠模型实验，作者使用IRF9抗体发现其在自身免疫疾病(如系统性红斑狼疮)患者的炎症组织中高表达，提示IRF9可能作为治疗靶点。
3. **"IRF9 Antibody-Based Detection of Transcriptional Regulation in Cancer"**
- **作者**: Gupta R, et al.
- **摘要**: 该研究采用IRF9抗体进行ChIP-seq分析，揭示了IRF9在肿瘤细胞中通过调控特定干扰素刺激基因(ISGs)影响肿瘤免疫逃逸的分子机制。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“IRF9 antibody”或“IRF9 function”，并筛选实验方法部分提及该抗体的研究。
The IRF9 (Interferon Regulatory Factor 9) antibody is a key tool for studying the function of IRF9. a transcription factor critical in mediating interferon (IFN) signaling. IRF9 is a member of the interferon regulatory factor family and forms the ISGF3 (IFN-Stimulated Gene Factor 3) complex with phosphorylated STAT1 and STAT2 upon IFN-α/β stimulation. This complex translocates to the nucleus, binds IFN-stimulated response elements (ISREs), and activates the transcription of interferon-stimulated genes (ISGs) involved in antiviral defense, immune regulation, and cell proliferation. Dysregulation of IRF9 has been linked to autoimmune diseases, viral infections, and cancer, making it a target for therapeutic research.
IRF9 antibodies are widely used in molecular and cellular studies to detect IRF9 expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. They are essential for investigating IFN signaling dynamics, JAK-STAT pathway activity, and cellular responses to pathogens or cytokines. Commercially available IRF9 antibodies are typically generated in rabbits or mice using immunogenic peptides corresponding to conserved regions of human or murine IRF9. Validation includes specificity tests using knockout cell lines or siRNA-mediated knockdown. Researchers prioritize antibodies with high affinity, minimal cross-reactivity, and compatibility with their experimental models to ensure reliable data in studies on innate immunity, cancer biology, or inflammatory disorders.
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