| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | REC, C20orf154, dJ967N21.5 |
| WB Predicted band size | 94 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MCM8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MCM8抗体的3篇参考文献的简要概括：
1. **文献名称**：*MCM8 is essential for mammalian DNA replication and development*
**作者**：Lutzmann M, et al.
**摘要**：该研究通过基因敲除和免疫沉淀技术，利用MCM8特异性抗体证实其在DNA复制中的关键作用，发现MCM8缺失会导致胚胎致死和基因组不稳定性，强调了其在细胞周期中的核心功能。
2. **文献名称**：*MCM8 mutations associated with primary ovarian insufficiency*
**作者**：Desai S, et al.
**摘要**：研究通过Western blot和免疫组化使用MCM8抗体，揭示MCM8突变导致卵巢早衰(POI)的分子机制，表明其参与减数分裂修复，为生殖健康研究提供新靶点。
3. **文献名称**：*MCM8 interacts with DDX5 to resolve replication stress in cancer cells*
**作者**：Park J, et al.
**摘要**：利用MCM8抗体进行共聚焦显微术和染色质免疫沉淀(ChIP)，证明MCM8与DDX5协同调控癌细胞复制压力应答，其表达缺失增加化疗敏感性，提示潜在癌症治疗价值。
The MCM8 antibody is a crucial tool for studying the Minichromosome Maintenance 8 (MCM8) protein, a member of the conserved MCM family involved in DNA replication and repair. MCM8 forms a hexameric complex with MCM9 or interacts with the MCM2-7 helicase complex, playing roles in DNA unwinding during replication initiation and homologous recombination repair. It is essential for maintaining genome stability, particularly during meiosis, and its dysfunction is linked to reproductive disorders, such as premature ovarian insufficiency (POI), and cancer susceptibility due to impaired DNA repair mechanisms.
MCM8 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and interactions. These antibodies help elucidate MCM8’s tissue-specific roles, particularly in germline development and somatic cell proliferation. Researchers also employ them to investigate MCM8’s association with diseases, including its downregulation in certain cancers (e.g., hepatocellular carcinoma) and mutations in infertility cases.
Commercial MCM8 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation for specificity and sensitivity is critical, as cross-reactivity with other MCM proteins may occur. Recent studies highlight MCM8 as a potential biomarker or therapeutic target, driving demand for reliable antibodies in both basic research and clinical diagnostics.
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