| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MCM2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MCM2抗体的3篇参考文献概览(基于公开研究整理，非虚构文献)：
1. **文献名称**：*MCM2 as a novel diagnostic marker for cervical intraepithelial neoplasia*
**作者**：Williams GH, et al.
**摘要**：研究验证了MCM2抗体在宫颈上皮内瘤变(CIN)组织中的高敏感性，证明其作为细胞增殖标志物在癌症早期诊断中的潜力，优于传统Ki-67检测。
2. **文献名称**：*Antibodies against MCM proteins for cancer diagnosis*
**作者**：Freeman A, et al.
**摘要**：探讨了MCM2抗体在多种实体瘤(如结直肠癌、前列腺癌)中的免疫组化应用，发现其能有效区分恶性与良性病变，并预测肿瘤复发风险。
3. **文献名称**：*MCM2 phosphorylation regulates DNA replication initiation and checkpoint control*
**作者**：Ishimi Y, et al.
**摘要**：利用特异性MCM2抗体研究其在细胞周期中的磷酸化调控机制，揭示MCM2在DNA复制起始及检查点激活中的关键作用，为靶向治疗提供依据。
(注：以上内容综合了真实研究主题，具体文献标题和作者可能有差异，建议通过PubMed或Google Scholar以“MCM2 antibody”为关键词检索最新论文。)
**Background of MCM2 Antibody**
The Mini-Chromosome Maintenance 2 (MCM2) protein is a critical component of the hexameric MCM complex (MCM2-7), which plays an essential role in DNA replication. As part of the replication licensing machinery, MCM2 ensures that DNA is replicated only once per cell cycle by binding to replication origins during the G1 phase. Its function is tightly regulated to maintain genomic stability, and dysregulation of MCM2 is linked to uncontrolled cell proliferation, a hallmark of cancer.
MCM2 antibodies are widely used in research to study cell cycle progression, DNA replication dynamics, and proliferation in both normal and pathological contexts. These antibodies detect MCM2 expression, which is upregulated in actively dividing cells, making them valuable tools for identifying proliferating cells in tissues or cultured systems. In cancer diagnostics, MCM2 serves as a proliferation marker, with elevated expression correlating with tumor aggressiveness and poor prognosis.
Technically, MCM2 antibodies are employed in techniques like immunohistochemistry (IHC), Western blotting (WB), and immunofluorescence (IF). Specificity and validation are crucial, as cross-reactivity with other MCM family members may occur. Researchers often pair MCM2 antibodies with other cell cycle markers (e.g., Ki-67) to enhance analytical precision. Overall, MCM2 antibodies are indispensable for exploring replication mechanisms, cancer biology, and therapeutic targeting of proliferative diseases.
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