| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NT4, NT5, NT-4, NT-5, NTF5, GLC10, GLC1O, NT-4/5 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NTF4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NTF4抗体的3篇示例文献概览(内容为虚构示例，仅供格式参考)：
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1. **"Development and validation of a high-affinity monoclonal antibody against human Neurotrophin-4 (NTF4)"**
*Author: Lee, J. et al.*
摘要：本研究报道了一种新型NTF4单克隆抗体的开发，通过杂交瘤技术筛选出高特异性抗体，并通过ELISA、Western blot和免疫组化验证其在人脑组织中的结合能力。该抗体可用于神经退行性疾病中NTF4蛋白表达的定量检测。
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2. **"NTF4 antibody-based therapeutic targeting in Alzheimer's disease models"**
*Author: Rodriguez, S. et al.*
摘要：文章探讨了NTF4抗体在阿尔茨海默病小鼠模型中的治疗潜力。实验表明，抗体可通过血脑屏障中和异常聚集的NTF4蛋白，减少神经元凋亡并改善认知功能，提示其作为神经保护剂的潜在应用。
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3. **"Structural mapping of NTF4 epitopes using phage display technology"**
*Author: Gupta, R. & Wang, T.*
摘要：通过噬菌体展示技术解析NTF4抗体的抗原表位，发现抗体主要结合于NTF4蛋白的β-折叠区域。该研究为优化抗体亲和力及设计靶向药物提供了结构基础。
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注：以上文献为模拟示例，实际引用需通过PubMed或Web of Science等数据库检索真实研究。如需具体文献推荐，建议补充研究领域或应用场景(如疾病、实验方法等)。
Neurotrophin-4 (NTF4), also known as NT-4 or NT-5 depending on species, is a member of the neurotrophin family of growth factors that play critical roles in neuronal survival, development, and plasticity. It binds to the tropomyosin receptor kinase B (TrkB) and the low-affinity p75 neurotrophin receptor, activating downstream signaling pathways involved in synaptic modulation, axon guidance, and cell differentiation. NTF4 is expressed in both the central and peripheral nervous systems and has been implicated in processes such as memory formation, pain regulation, and recovery from neural injury. Dysregulation of NTF4 signaling has been linked to neurodegenerative diseases (e.g., Alzheimer’s), mood disorders, and neuropathic pain.
Antibodies targeting NTF4 are essential tools for studying its expression, localization, and function in biological systems. They are widely used in techniques like Western blotting, immunohistochemistry, and ELISA to quantify protein levels or visualize distribution in tissues. Commercial NTF4 antibodies are typically raised in hosts such as rabbits or mice using recombinant protein fragments or synthetic peptides. However, challenges remain in ensuring specificity due to high homology among neurotrophin family members. Recent research emphasizes the need for rigorous validation via knockout controls or neutralization assays. Such antibodies have advanced understanding of NTF4's role in neuroprotection and its potential as a therapeutic target, particularly in conditions involving neuronal degeneration or synaptic dysfunction.
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