| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PYY4 |
| WB Predicted band size | 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NPY |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NPY抗体的示例参考文献(注：以下文献信息为示例性质，实际引用请通过学术数据库核实)：
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1. **文献名称**: *"Characterization of a Novel Neuropeptide Y Antibody for Immunohistochemical Analysis in Rodent Brain"*
**作者**: Smith A, et al.
**摘要**: 本研究开发并验证了一种高特异性兔源多克隆NPY抗体，通过免疫组化技术在小鼠大脑组织中检测NPY分布。结果显示该抗体可特异性标记下丘脑及杏仁核区域的NPY神经元，且无交叉反应性。
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2. **文献名称**: *"NPY Antibody-Based Detection of Obesity-Related Neuropeptide Alterations in Human Plasma"*
**作者**: Chen L, et al.
**摘要**: 利用商业化NPY抗体建立ELISA检测方法，分析肥胖患者血浆中NPY水平变化。研究发现肥胖人群NPY浓度显著升高，提示其与代谢紊乱的潜在关联。
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3. **文献名称**: *"Differential Expression of Neuropeptide Y in Cardiac Tissue: Validation of a Monoclonal Antibody for Western Blot"*
**作者**: Gonzalez R, et al.
**摘要**: 验证一种新型NPY单克隆抗体在心肌缺血模型中的应用，通过蛋白质印迹和免疫荧光技术证实NPY在缺血心脏组织中的表达上调，提示其在心血管疾病中的作用。
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4. **文献名称**: *"Comparative Study of NPY Antibody Specificity Across Species: Implications for Translational Research"*
**作者**: Tanaka K, et al.
**摘要**: 比较多种NPY抗体对小鼠、大鼠及人类样本的反应性，发现部分抗体存在种属交叉反应差异，强调抗体选择对跨物种研究的重要性。
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建议通过PubMed或Web of Science检索关键词“NPY antibody”或“Neuropeptide Y immunoassay”获取真实文献。
Neuropeptide Y (NPY) antibodies are essential tools in neuroscience and endocrine research for detecting and quantifying NPY, a 36-amino-acid neuropeptide widely distributed in the central and peripheral nervous systems. NPY, part of the pancreatic polypeptide family, plays critical roles in regulating energy balance, stress responses, emotional behavior, and cardiovascular function. It is highly expressed in brain regions like the hypothalamus, amygdala, and brainstem, as well as in sympathetic neurons. Dysregulation of NPY signaling is linked to conditions such as obesity, anxiety, depression, epilepsy, and hypertension.
NPY antibodies are typically produced by immunizing host animals (e.g., rabbits, mice) with synthetic NPY peptides or recombinant proteins. These antibodies vary in specificity, targeting distinct epitopes (e.g., C-terminal or N-terminal regions), and are available as monoclonal or polyclonal forms. Validation methods, including Western blotting, immunohistochemistry (IHC), and ELISA, ensure their reliability in detecting NPY in tissues, cells, or biological fluids. Applications span basic research—such as mapping NPY expression in neural circuits—to clinical studies investigating NPY's role in metabolic or psychiatric disorders. Challenges include cross-reactivity with related peptides (e.g., peptide YY) and ensuring batch consistency. Advanced antibody engineering (e.g., recombinant antibodies) continues to enhance specificity and reproducibility, supporting NPY-related mechanistic and therapeutic research.
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