| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NRF2; HEBP1; Nrf-2; IMDDHH |
| WB Predicted band size | 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NFE2L2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是与NFE2L2(Nrf2)抗体相关的3篇文献摘要示例：
1. **文献名称**：*Regulation of the Nrf2-Keap1 Antioxidant Response Pathway*
**作者**：Yamamoto M, Kensler TW, Motohashi H
**摘要**：该综述探讨了Nrf2在抗氧化应激中的核心作用，详细描述了Nrf2与Keap1的相互作用及调控机制。文献提到通过特异性抗体(如ChIP-seq和Western blot)验证Nrf2的核转位及其靶基因激活，为抗氧化治疗研究提供方法学支持。
2. **文献名称**：*Distinct mechanisms of Nrf2 regulation in physiological and pathological contexts*
**作者**：Suzuki T, Yamamoto M
**摘要**：研究比较了Nrf2在正常生理和癌症中的不同调控机制，利用Nrf2特异性抗体进行免疫组化和蛋白质印迹分析，揭示了其在氧化损伤保护和肿瘤耐药性中的双重作用，强调抗体选择对结果准确性的影响。
3. **文献名称**：*Antibody-based profiling of Nrf2 activation in neurodegenerative disease models*
**作者**：Johnson DA, Johnson JA
**摘要**：本文通过Nrf2抗体检测阿尔茨海默病和帕金森病模型中Nrf2的激活水平，结合免疫荧光和ELISA技术，证明其神经保护作用，并验证了抗体的特异性在跨物种研究中的适用性。
(注：以上文献为示例，实际引用时需核实真实存在的论文及作者信息。)
The NFE2L2 antibody is a crucial tool in studying the nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor encoded by the NFE2L2 gene. Nrf2 plays a central role in cellular defense mechanisms against oxidative stress and electrophilic insults by regulating the expression of antioxidant response element (ARE)-driven genes. Under basal conditions, Nrf2 is sequestered in the cytoplasm by its inhibitor, Keap1. and targeted for proteasomal degradation. However, oxidative stress or exposure to chemopreventive agents disrupts this interaction, allowing Nrf2 to translocate to the nucleus, dimerize with small Maf proteins, and activate cytoprotective genes involved in detoxification, redox balance, and metabolism.
NFE2L2 antibodies are widely used in research to detect Nrf2 protein expression, localization, and activation status in various disease models, including cancer, neurodegenerative disorders, and inflammatory conditions. Dysregulation of the Nrf2 pathway is implicated in both pathogenesis and chemoresistance, making these antibodies essential for mechanistic studies. They are employed in techniques like Western blotting, immunohistochemistry, immunofluorescence, and ChIP assays. Specificity for Nrf2 is critical due to structural similarities with other Nrf family members. Researchers also utilize these antibodies to explore therapeutic strategies targeting Nrf2 activation or inhibition, aiming to modulate oxidative stress responses in diseases.
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