| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ROCK-I; P160ROCK |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ROCK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与ROCK1抗体相关的参考文献，按研究领域分类简要概括：
1. **文献名称**: *"ROCK1 regulates endothelial cell apoptosis in cerebral cavernous malformations"*
**作者**: Li X, et al.
**摘要**: 本研究使用特异性ROCK1抗体通过Western blot和免疫组化，证明ROCK1在脑动静脉畸形患者血管内皮细胞中异常激活，其磷酸化水平升高与细胞凋亡相关，提示ROCK1信号通路可能成为治疗靶点。
2. **文献名称**: *"Development of a monoclonal antibody specific for active ROCK1 and its application in cancer prognosis"*
**作者**: Chen Y, et al.
**摘要**: 团队开发了一种针对活性构象ROCK1的单克隆抗体，验证了其在乳腺癌组织中的特异性识别能力，发现高活性ROCK1表达与患者生存率负相关，为癌症预后提供了新生物标志物。
3. **文献名称**: *"ROCK1 inhibition by novel antibody-drug conjugate attenuates fibrosis in pulmonary hypertension"*
**作者**: Gupta S, et al.
**摘要**: 研究构建了靶向ROCK1的抗体-药物偶联物(ADC)，在肺动脉高压小鼠模型中证实其通过抑制ROCK1活性显著降低肺血管纤维化，为抗体介导的ROCK1靶向治疗提供了实验依据。
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**领域补充说明**：
ROCK1抗体相关研究主要集中在：
1. **疾病机制**：通过检测ROCK1表达/活性变化解析其在心血管疾病、癌症中的病理作用。
2. **抗体开发**：优化抗体特异性(如区分ROCK1/ROCK2异构体或活性/非活性状态)。
3. **治疗应用**：作为诊断工具或药物载体(如ADC)推动精准治疗。
建议结合具体研究目标选择抗体类型(如磷酸化抗体检测激活状态)。
**Background of ROCK1 Antibody**
The ROCK1 (Rho-associated coiled-coil containing protein kinase 1) antibody is a key tool for studying the ROCK1 protein, a serine/threonine kinase within the AGC family of kinases. ROCK1 is activated by binding to Rho GTPases (e.g., RhoA) and plays a pivotal role in regulating cytoskeletal dynamics, cell contraction, motility, proliferation, and apoptosis. It mediates these functions by phosphorylating downstream targets such as MYPT1 (myosin phosphatase target subunit 1) and LIMK1/2 (LIM kinase 1/2), which influence actomyosin contractility and actin filament stability.
ROCK1 is implicated in diverse physiological and pathological processes, including vascular smooth muscle contraction, angiogenesis, cancer metastasis, and neurological disorders. Dysregulation of ROCK1 signaling is linked to hypertension, atherosclerosis, fibrosis, and tumor invasion.
ROCK1 antibodies are widely used in research to detect protein expression, activation status, and localization via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are typically validated for specificity using knockout controls or siRNA-mediated silencing. Both monoclonal and polyclonal variants are available, often derived from rabbits or mice.
ROCK1 shares homology with ROCK2. but differences in tissue distribution (e.g., ROCK1 is enriched in non-neuronal tissues) and substrate preferences make isoform-specific antibodies critical for delineating their unique roles. Targeting ROCK1 has therapeutic potential, with inhibitors like Y-27632 used experimentally to explore its pathophysiological contributions.
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