| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ROCK-II |
| WB Predicted band size | 161 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ROCK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ROCK2抗体的3篇示例参考文献(注：文献信息为模拟示例，仅供参考)：
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1. **文献名称**：*"ROCK2-Specific Antibody Reveals Its Role in Cancer Cell Invasion via Cytoskeletal Remodeling"*
**作者**：Li, X. et al.
**摘要**：本研究利用ROCK2特异性抗体，通过免疫印迹和免疫荧光技术，揭示了ROCK2在乳腺癌细胞中通过调节肌动蛋白骨架促进侵袭转移的机制，并证明其与患者预后不良相关。
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2. **文献名称**：*"Targeting ROCK2 with a Monoclonal Antibody Attenuates Neuroinflammation in Alzheimer's Disease Models"*
**作者**：Smith, J. & Patel, R.
**摘要**：文章报道了一种新型抗ROCK2单克隆抗体的开发，并在阿尔茨海默病小鼠模型中验证其效果。结果显示，该抗体通过抑制ROCK2活性减少tau蛋白过度磷酸化及神经炎症反应。
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3. **文献名称**：*"ROCK2 Inhibition by a Neutralizing Antibody Improves Cardiac Fibrosis in Hypertensive Rats"*
**作者**：Yamamoto, K. et al.
**摘要**：研究使用ROCK2中和抗体干预高血压模型大鼠，发现其能显著降低心肌纤维化标志物(如胶原沉积)，提示ROCK2抗体在心血管疾病治疗中的潜在应用价值。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词(如“ROCK2 antibody”、“ROCK2 inhibitor”)，并筛选近年高影响力期刊的研究(如*Nature Cell Biology*、*Journal of Biological Chemistry*等)。
The Rho-associated coiled-coil kinase 2 (ROCK2) antibody is a crucial tool for studying the ROCK2 protein, a serine/threonine kinase belonging to the AGC family of kinases. ROCK2. along with its isoform ROCK1. plays a pivotal role in regulating cytoskeletal dynamics, cell adhesion, migration, and apoptosis through its interaction with Rho GTPases, particularly RhoA. It mediates downstream signaling by phosphorylating substrates such as myosin light chain (MLC) and LIM kinase, influencing actomyosin contractility and stress fiber formation.
ROCK2 is implicated in diverse physiological and pathological processes, including cardiovascular remodeling, neuronal degeneration, cancer metastasis, and fibrosis. Its overexpression or hyperactivity has been linked to hypertension, atherosclerosis, and glaucoma. The ROCK2 antibody enables detection and quantification of ROCK2 expression in tissues and cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. It also aids in elucidating ROCK2's spatial-temporal localization and activation status under experimental or disease conditions.
Researchers frequently use ROCK2-specific antibodies to validate gene silencing (e.g., siRNA/CRISPR) or assess pharmacological inhibition by compounds like fasudil or Y-27632. As ROCK2-selective inhibitors gain traction in drug development, high-specificity antibodies are essential for distinguishing ROCK2 from ROCK1. which share ~65% homology but exhibit distinct tissue distributions and functions. This underscores the antibody's importance in both basic research and therapeutic targeting of ROCK2-associated pathways.
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