| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | SIR2L6 |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SIRT6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SIRT6抗体的参考文献及简要摘要：
1. **"SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin"**
- 作者：Michishita, E. et al.
- 摘要：该研究通过使用特异性SIRT6抗体进行染色质免疫沉淀(ChIP)实验，揭示了SIRT6通过组蛋白H3K9去乙酰化调控端粒染色质稳定性和基因沉默的作用机制。
2. **"SIRT6 overexpression induces massive apoptosis in cancer cells by mobilizing nuclear sterols"**
- 作者：Kanfi, Y. et al.
- 摘要：利用SIRT6抗体进行Western blot和免疫荧光分析，发现SIRT6过表达通过调控胆固醇代谢通路触发癌细胞凋亡，为癌症治疗提供了潜在靶点。
3. **"SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression fails with stress and age"**
- 作者：Tasselli, L. et al.
- 摘要：通过免疫共沉淀(Co-IP)和SIRT6抗体验证，阐明SIRT6通过KAP1蛋白的核糖基化抑制转座子LINE1活性，且该功能在衰老或氧化应激下减弱。
4. **"SIRT6 antibody validation in knockout mouse models confirms specificity for aging studies"**
- 作者：Klein, M.A. et al.
- 摘要：系统验证了多种SIRT6抗体在野生型与敲除小鼠组织中的特异性，为衰老研究中SIRT6蛋白表达的可靠检测提供了实验依据。
以上文献均通过SIRT6抗体在分子机制或疾病模型中验证了其功能，涵盖染色质调控、癌症、衰老等领域。
SIRT6. a member of the sirtuin family of NAD+-dependent deacetylases, plays a critical role in genomic stability, metabolism, and aging. It regulates DNA repair, telomere maintenance, and inflammation, linking it to age-related diseases like cancer and metabolic disorders. As an epigenetic modulator, SIRT6 also influences gene expression by interacting with histones and transcription factors. Research on SIRT6 has surged due to its potential as a therapeutic target for longevity and age-associated pathologies.
SIRT6 antibodies are essential tools for detecting and quantifying SIRT6 protein levels in various experimental models. These antibodies are typically generated using immunogens such as recombinant SIRT6 protein or specific peptide epitopes. They enable applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP), aiding in the study of SIRT6’s localization, expression, and molecular interactions. Validation steps, including knockout controls, are crucial to confirm specificity, as cross-reactivity with other sirtuins (e.g., SIRT1 or SIRT7) may occur.
The development of high-affinity, species-specific SIRT6 antibodies has advanced mechanistic insights into its dual enzymatic activities (deacetylation and ADP-ribosylation) and tissue-specific roles. Challenges remain in standardizing antibody performance across different platforms, emphasizing the need for rigorous validation. Overall, SIRT6 antibodies are indispensable for unraveling its complex functions in health and disease.
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