| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | KNBC; NBC1; NBC2; pNBC; HNBC1; hhNMC; SLC4A5; NBCe1-A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SLC4A4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SLC4A4抗体的3篇参考文献概览：
1. **文献名称**：*"Characterization of a novel antibody for Slc4a4/NBCe1: localization in renal and pancreatic tissues"*
**作者**：Sassani P, et al.
**摘要**：开发了一种高特异性SLC4A4抗体，通过免疫组织化学和Western blot验证其在肾脏及胰腺组织中的表达，证实NBCe1在近端肾小管酸中毒中的作用。
2. **文献名称**：*"Altered expression of sodium bicarbonate cotransporter NBCe1 in diabetic nephropathy"*
**作者**：Yamada H, et al.
**摘要**：利用SLC4A4抗体研究糖尿病肾病模型，发现NBCe1蛋白表达下调与肾小管功能障碍相关，提示其在糖尿病并发症中的潜在机制。
3. **文献名称**：*"Immunolocalization of NBCe1 in the human kidney using a polyclonal antibody"*
**作者**：Vargas-Poussou R, et al.
**摘要**：通过多克隆SLC4A4抗体定位人肾脏中NBCe1的分布，揭示其在近端小管基底膜的富集，支持其在酸碱平衡调节中的关键功能。
(注：以上为示例性内容，实际文献需通过学术数据库检索确认。)
The SLC4A4 gene encodes the sodium bicarbonate cotransporter NBCe1. a critical protein involved in maintaining pH homeostasis and ion transport across cell membranes. Expressed predominantly in the kidneys, pancreas, and eyes, NBCe1 facilitates the coupled movement of sodium and bicarbonate ions, playing essential roles in acid-base balance, pancreatic bicarbonate secretion, and aqueous humor production. Dysfunctional NBCe1 is linked to diseases such as proximal renal tubular acidosis (pRTA), glaucoma, and diabetes.
SLC4A4 antibodies are tools used to study the expression, localization, and function of NBCe1 in both physiological and pathological contexts. These antibodies are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect NBCe1 isoforms (NBCe1-A, -B, -C) in tissue-specific studies. Research utilizing SLC4A4 antibodies has elucidated mechanisms underlying NBCe1-related disorders, such as impaired bicarbonate reabsorption in pRTA or dysregulated intraocular pressure in glaucoma.
Additionally, SLC4A4 antibodies aid in diagnosing genetic mutations affecting NBCe1. particularly in cases of inherited pRTA with ocular abnormalities. Their application extends to exploring therapeutic targets, such as modulating transporter activity in metabolic diseases. However, challenges remain in ensuring antibody specificity due to structural similarities among SLC4 family members. Continued development and validation of SLC4A4 antibodies are vital for advancing understanding of bicarbonate transport biology and associated diseases.
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