Mouse Hepatocyte Growth Factor Receptor(C-MET/HGFR)ELISA Kit

货号: IPD-E13492m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠肝细胞生长因子受体(c-MET/HGFR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MET含量。MET是一种原癌基因编码的受体酪氨酸激酶。其异常激活与肿瘤的发生、发展、转移及预后密切相关。研究机制主要聚焦于MET信号通路，阻断该通路可抑制肿瘤细胞增殖、存活和迁移，为肿瘤治疗提供了潜在的靶点策略。试剂盒检测范围为0.078 ng/mL-5 ng/mL，灵敏度为0.02 ng/mL。适用于小鼠疾病模型中肿瘤微环境研究、肝脏再生机制探索以及靶向药物干预实验的疗效评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Met ELISA Kit; Hepatocyte growth factor receptor ELISA Kit; HGF receptor ELISA Kit; EC 2.7.10.1 ELISA Kit; HGF/SF receptor ELISA Kit; Proto-oncogene c-Met ELISA Kit; Scatter factor receptor ELISA Kit; SF receptor ELISA Kit; Tyrosine-protein kinase Met ELISA Kit
缩写：
MET
Uniprot No.：
P16056
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.078 ng/mL-5 ng/mL
灵敏度：
0.02 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)639.988613.434633.318588.302625.563621.144SD0.0350.0400.0360.0450.0480.052CV(%)4.6985.5484.8716.4476.5577.143

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-MET/HGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-96
1:2	Average %	102
	Range %	96-107
1:4	Average %	91
	Range %	87-96
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of mouse C-MET/HGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	104	92-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
5	2.723	2.854	2.789	2.701
2.5	1.856	1.987	1.922	1.834
1.25	1.219	1.264	1.242	1.154
0.625	0.715	0.732	0.724	0.636
0.312	0.399	0.386	0.393	0.305
0.156	0.248	0.255	0.252	0.164
0.078	0.158	0.162	0.160	0.072
0	0.087	0.088	0.088