| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | KCC1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SLC12A4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SLC12A4抗体的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: *SLC12A4 mediates chloride transport in activated T cells and regulates immune response*
**作者**: Müller A, et al.
**摘要**: 研究利用SLC12A4特异性抗体证实其在T细胞活化后膜定位，通过调控Cl⁻转运影响细胞迁移和炎症反应，为自身免疫疾病治疗提供新靶点。
2. **文献名称**: *Expression profiling of SLC12A4 in colorectal cancer using a novel monoclonal antibody*
**作者**: Chen L, et al.
**摘要**: 开发针对SLC12A4胞外结构域的单克隆抗体，通过免疫组化发现其在结肠癌组织中高表达，与患者预后不良相关，提示其作为肿瘤生物标志物的潜力。
3. **文献名称**: *Functional characterization of SLC12A4 variants in erythrocyte volume regulation*
**作者**: Tanaka K, et al.
**摘要**: 采用SLC12A4抗体进行红细胞膜蛋白检测，结合基因敲除模型，揭示SLC12A4突变导致红细胞离子失衡，与遗传性溶血性贫血相关。
4. **文献名称**: *SLC12A4 antibody-based inhibition suppresses neuronal apoptosis in ischemic stroke*
**作者**: Gupta R, et al.
**摘要**: 研究通过抗体阻断SLC12A4的离子转运功能，发现其可减轻脑缺血后神经元内Cl⁻超载，从而抑制凋亡通路，为中风治疗提供新策略。
注：以上内容为模拟生成，实际文献需通过PubMed或Google Scholar检索关键词如“SLC12A4 antibody”、“SLC12A4 function”获取。
The SLC12A4 antibody is a research tool designed to detect the SLC12A4 protein, encoded by the SLC12A4 gene, which belongs to the solute carrier family 12 (SLC12) of cation-chloride cotransporters. SLC12A4. also known as KCC1 (K-Cl cotransporter 1), facilitates the electroneutral transport of potassium and chloride ions across cell membranes, playing a critical role in cellular ion homeostasis and volume regulation. This transmembrane protein is expressed in various tissues, including the kidney, brain, and red blood cells, where it contributes to chloride efflux, particularly under hypotonic conditions.
Antibodies targeting SLC12A4 are widely used in biomedical research to investigate its expression patterns, subcellular localization, and functional roles in physiological and pathological contexts. For example, studies have linked SLC12A4 to neurological disorders, such as epilepsy, due to its involvement in modulating neuronal chloride concentrations and GABAergic signaling. Additionally, dysregulation of SLC12A4 has been implicated in cancer progression, hypertension, and renal dysfunction.
These antibodies are typically validated in applications like Western blotting, immunohistochemistry, and immunofluorescence, often using samples from cell lines, animal models, or human tissues. Researchers rely on SLC12A4 antibodies to explore its interaction with regulatory kinases (e.g., WNK kinases) and its response to osmotic stress or pharmacological modulators. Ensuring antibody specificity via knockout controls or peptide blocking is essential to avoid cross-reactivity with related transporters like KCC3 or KCC4. Overall, SLC12A4 antibodies are vital for advancing understanding of ion transport mechanisms and their disease associations.
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