| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | CCDC99 |
| WB Predicted band size | 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SPDL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SPDL1抗体的3篇文献摘要信息(注：文献为模拟示例，实际引用需核实)：
1. **文献名称**：*SPDL1 regulates mitotic spindle architecture and chromosome segregation through Aurora B kinase interaction*
**作者**：Li, X., et al.
**摘要**：本研究利用特异性SPDL1抗体进行免疫沉淀和免疫荧光实验，揭示了SPDL1通过与Aurora B激酶相互作用调控纺锤体组装及染色体分离的机制，为理解其在细胞分裂中的功能提供依据。
2. **文献名称**：*Development of a monoclonal antibody targeting SPDL1 for detection of mitotic errors in cancer cells*
**作者**：Zhang, Y., et al.
**摘要**：文章报道了一种新型SPDL1单克隆抗体的开发，验证其在Western blot和免疫组化中的高特异性，并应用于多种癌细胞系，发现SPDL1表达异常与有丝分裂错误显著相关。
3. **文献名称**：*SPDL1 as a potential biomarker in triple-negative breast cancer: Insights from antibody-based proteomic analysis*
**作者**：Wang, H., et al.
**摘要**：通过抗体验证，研究证实SPDL1在三阴性乳腺癌组织中高表达，且与患者预后不良相关，提示其可能作为治疗靶点或诊断标志物。
如需具体文献，建议通过PubMed或Google Scholar以“SPDL1 antibody”为关键词检索最新研究。
SPDL1 (Spindle Apparatus Coiled-Coil Protein 1), also known as CCDC99 or SPATA24. is a critical protein involved in mitotic regulation, particularly during the metaphase-to-anaphase transition. It localizes to the spindle apparatus and plays a role in ensuring proper chromosome segregation by contributing to the spindle assembly checkpoint (SAC), a surveillance mechanism that prevents premature anaphase onset until all chromosomes are correctly attached to microtubules. SPDL1 interacts with components of the SAC, such as the ROD-ZW10-ZWILCH (RZZ) complex and dynein-dynactin motors, facilitating the removal of checkpoint proteins from kinetochores once attachments are stabilized. Dysregulation of SPDL1 is implicated in chromosomal instability, a hallmark of cancer and developmental disorders.
SPDL1 antibodies are essential tools for studying its expression, localization, and function in cell division. They are widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to investigate SPDL1’s role in mitotic fidelity, cancer biology, and potential therapeutic targeting. Recent studies highlight SPDL1’s overexpression in certain cancers, linking it to tumor progression and poor prognosis. Antibodies targeting specific epitopes of SPDL1 also aid in exploring its interaction networks and post-translational modifications. As research advances, SPDL1 antibodies may contribute to developing strategies to modulate SAC activity, offering avenues for cancer treatment by exploiting mitotic vulnerabilities in malignant cells.
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