| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MUTED |
| Uniprot No | Q8TDH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-187aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSGGGTE TPVGCEAAPG GGSKKRDSLG TAGSAHLIIK DLGEIHSRLL DHRPVIQGET RYFVKEFEEK RGLREMRVLE NLKNMIHETN EHTLPKCRDT MRDSLSQVLQ RLQAANDSVC RLQQREQERK KIHSDHLVAS EKQHMLQWDN FMKEQPNKRA EVDEEHRKAM ERLKEQYAEM EKDLAKFSTF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MUTED重组蛋白的虚构参考文献示例(仅供格式参考，非真实文献)：
1. **文献名称**：*Structural and Functional Characterization of the MUTED Protein in Lysosome-Related Organelles*
**作者**：Chen L, et al.
**摘要**：本研究通过重组表达MUTED蛋白，解析了其与BLOC-1复合体其他亚基的相互作用机制，证明其在溶酶体相关细胞器(LROs)生物发生中的关键作用，并揭示了MUTED缺失导致细胞内黑色素颗粒运输异常的分子机制。
2. **文献名称**：*Recombinant MUTED Facilitates Cargo Sorting in Endosomal Pathways*
**作者**：Martinez R, et al.
**摘要**：利用大肠杆菌系统成功表达并纯化重组MUTED蛋白，结合细胞模型发现其通过调控内体分选复合体(ESCRT)的募集，影响跨膜蛋白的溶酶体靶向运输，为Hermansky-Pudlak综合征的病理机制提供新见解。
3. **文献名称**：*MUTED Knockout Mice Exhibit Defects in Platelet Dense Granule Formation*
**作者**：Sato K, et al.
**摘要**：通过构建MUTED基因敲除小鼠模型，发现重组MUTED蛋白回补可恢复血小板致密颗粒的生成缺陷，证实其在颗粒蛋白组装中的特异性作用，并关联了BLOC-1复合体功能障碍与遗传性出血性疾病的关系。
4. **文献名称**：*Biochemical Analysis of MUTED in Melanosome Biogenesis*
**作者**：Nguyen T, et al.
**摘要**：通过体外重组MUTED蛋白与TYRP1的互作实验，揭示其参与黑色素细胞中黑素小体成熟过程的分子通路，为皮肤色素沉着异常疾病的治疗提供潜在靶点。
注：以上文献为模拟内容，实际研究中请通过PubMed或Google Scholar等平台检索真实文献。
The MUTED protein, encoded by the *MUTED* gene, is a component of the Biogenesis of Lysosome-Related Organelles Complex-1 (BLOC-1), which plays a critical role in intracellular membrane trafficking and lysosome-related organelle biogenesis. BLOC-1 facilitates cargo sorting and transport to lysosomes and specialized organelles like melanosomes and platelet dense granules. MUTED, along with other BLOC-1 subunits (e.g., BLOS1. BLOS2. and pallidin), contributes to these processes by interacting with vesicular trafficking machinery and cytoskeletal elements. Dysregulation of BLOC-1 function is linked to disorders such as Hermansky-Pudlak syndrome (HPS), characterized by albinism, bleeding disorders, and lysosomal defects.
Recombinant MUTED protein is engineered for in vitro studies to dissect its structural and functional roles in BLOC-1-mediated pathways. Produced via expression systems like *E. coli* or mammalian cells, it retains key domains for protein-protein interactions, enabling researchers to explore its binding partners, post-translational modifications, and regulatory mechanisms. Recombinant MUTED is vital for reconstituting BLOC-1 complexes, studying trafficking defects in HPS models, and screening therapeutic compounds targeting lysosomal disorders.
Research using recombinant MUTED has advanced understanding of organelle biogenesis and intracellular transport, with implications for neurodegenerative diseases (e.g., Parkinson’s) and immune cell function. Its application in structural studies (e.g., crystallography or cryo-EM) also aids in mapping BLOC-1 architecture. Overall, MUTED recombinant protein serves as a tool to unravel lysosomal biology and develop treatments for trafficking-related diseases.
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