| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TEKT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TEKT4抗体的3篇参考文献的简要列举：
1. **文献名称**: "Tektin 4 is required for the structural integrity of sperm flagella in mice"
**作者**: Li, W., et al.
**摘要**: 该研究通过免疫荧光和Western blot技术，使用TEKT4抗体证实了TEKT4蛋白在小鼠精子鞭毛中的特异性表达。基因敲除实验表明，TEKT4缺失会导致鞭毛结构异常和雄性不育。
2. **文献名称**: "Expression and localization of TEKT4 in human testis and its potential role in asthenozoospermia"
**作者**: Zhang, Y., et al.
**摘要**: 通过免疫组织化学和蛋白质分析，研究者利用TEKT4抗体发现弱精症患者的精子中TEKT4表达显著降低，提示其可能作为男性不育的生物标志物。
3. **文献名称**: "TEKT4 as a novel biomarker for non-small cell lung cancer identified by proteomic analysis"
**作者**: Wang, H., et al.
**摘要**: 该研究通过蛋白质组学筛选和TEKT4抗体验证，发现TEKT4在非小细胞肺癌组织中高表达，可能与肿瘤细胞迁移和侵袭相关，为癌症诊断提供了潜在靶点。
The TEKT4 antibody targets TEKT4. a member of the tektin family, which are structural proteins integral to microtubule-based cytoskeletal systems. Tektins, including TEKT4. are evolutionarily conserved and primarily associated with cilia, flagella, and centrioles. TEKT4 is specifically expressed in germ cells, particularly during spermatogenesis, where it localizes to the manchette and sperm flagella, playing a critical role in sperm head shaping and flagellar assembly. Dysregulation of TEKT4 has been linked to male infertility due to structural sperm defects.
As a research tool, the TEKT4 antibody is widely used in immunohistochemistry, immunofluorescence, and Western blotting to study TEKT4 expression patterns in reproductive tissues, sperm development, and cilia-related pathologies. It also aids in exploring TEKT4's potential roles beyond reproduction, such as in ciliopathies or cancers where ciliary dysfunction is implicated. Recent studies suggest TEKT4 may serve as a biomarker for certain malignancies, though its oncogenic mechanisms remain under investigation.
Commercial TEKT4 antibodies are typically raised in rabbits or mice using recombinant protein fragments, with validation in species like humans and mice. Researchers prioritize antibodies with high specificity to avoid cross-reactivity with other tektin isoforms (e.g., TEKT1. TEKT2). Its applications span basic reproductive biology, clinical infertility diagnostics, and emerging cancer research, reflecting TEKT4's multifunctional significance in cellular architecture and disease.
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