| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | alpha4GnT |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human A4GNT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于A4GNT抗体的3篇文献示例(信息基于公开研究内容概括，非真实文献)：
1. **文献名称**：*Alpha1.4-N-acetylglucosaminyltransferase (A4GNT) regulates gastric cancer progression via glycosylation of MUC5AC*
**作者**：Taniuchi K, et al.
**摘要**：研究揭示了A4GNT在胃腺癌中通过糖基化修饰MUC5AC黏蛋白调控肿瘤侵袭性的机制，并开发了特异性A4GNT抗体用于组织样本中酶活性检测，证明其表达与患者预后相关。
2. **文献名称**：*A4GNT deficiency alters gastric mucosal homeostasis and promotes tumorigenesis in mice*
**作者**：Nomura M, et al.
**摘要**：通过构建A4GNT基因敲除小鼠模型，结合A4GNT抗体进行免疫组化分析，发现A4GNT缺失导致胃黏膜屏障功能受损，并增加化学诱导胃癌的易感性。
3. **文献名称**：*Development of a monoclonal antibody against human A4GNT for diagnostic applications in gastrointestinal diseases*
**作者**：Yoshimura T, et al.
**摘要**：报道了一种高特异性抗A4GNT单克隆抗体的制备与验证，该抗体可用于ELISA和免疫荧光技术，显著提升胃癌及癌前病变的诊断准确性。
注：以上文献为示例，实际研究请通过PubMed或学术数据库查询最新成果。
The A4GNT antibody targets the α1.4-N-acetylglucosaminyltransferase (A4GNT) enzyme, a glycosyltransferase critical for synthesizing unique O-glycan structures in the gastrointestinal tract. A4GNT catalyzes the transfer of GlcNAc residues via α1.4-linkages to core 1 mucin-type O-glycans, forming terminal α1.4-GlcNAc-capped structures. These glycans are predominantly expressed in gastric gland mucous cells and play a key role in mucosal defense.
A4GNT's activity is particularly associated with the formation of the "glycoform" recognized by the pathogen Helicobacter pylori, which binds to α1.4-GlcNAc residues during gastric colonization. Reduced A4GNT expression has been linked to gastric pathologies, including chronic gastritis and gastric cancer, as impaired α1.4-GlcNAc production compromises mucosal barrier function. A4GNT antibodies are valuable tools for studying mucin glycosylation patterns, host-pathogen interactions, and gastric disease mechanisms. They enable detection of A4GNT protein localization in tissues, assessment of enzyme expression levels, and functional studies exploring glycosylation's role in mucosal homeostasis. Recent research also investigates A4GNT's potential as a biomarker for early gastric cancer detection and its involvement in intestinal metaplasia progression.
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