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产品描述：人脂蛋白磷脂酶A2(Lp-PLA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLA2G7含量。PLA2G7 是一种具有重要研究价值的靶点。它参与细胞内磷脂代谢等生理过程，在炎症反应、心血管疾病等病理机制中发挥作用。对其研究机制主要聚焦于它在相关疾病发生发展中的信号传导和调控作用，有望为相关疾病治疗提供新方向。试剂盒检测范围为1.56 ng/mL-100 ng/mL，灵敏度为0.39 ng/mL。心血管疾病模型研究、炎症相关分子机制探索、药物干预效果评价本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Platelet-activating factor acetylhydrolase precursor ELISA Kit; 1 alkyl 2 acetylglycerophosphocholine esterase ELISA Kit; 1-alkyl-2-acetylglycerophosphocholine esterase ELISA Kit; 2 acetyl 1 alkylglycerophosphocholine esterase ELISA Kit; 2-acetyl-1-alkylglycerophosphocholine esterase ELISA Kit; EC 3.1.1.47 PAF acetylhydrolase PAF 2-acylhydrolase LDL associated phospholipase A2 ELISA Kit; Group VIIA phospholipase A2 ELISA Kit; Group-VIIA phospholipase A2 ELISA Kit; gVIIA PLA2 ELISA Kit; gVIIA-PLA2 ELISA Kit; LDL associated phospholipase A2 ELISA Kit; LDL PLA(2) ELISA Kit; LDL PLA2 ELISA Kit; LDL-associated phospholipase A2 ELISA Kit; LDL-PLA(2) ELISA Kit; Lipoprotein associated phospholipase A2 ELISA Kit; LP PLA2 ELISA Kit; PAF 2 acylhydrolase ELISA Kit; PAF 2 acylhydrolase LDL associated phospholipase A2 ELISA Kit; PAF 2-acylhydrolase ELISA Kit; PAF acetylhydrolase ELISA Kit; PAFA_HUMAN ELISA Kit; PAFAD ELISA Kit; PAFAH ELISA Kit; Phospholipase A2, group VII (platelet activating factor acetylhydrolase, plasma) ELISA Kit; PLA2G7 ELISA Kit; Platelet activating factor acetylhydrolase 2 ELISA Kit; Platelet activating factor acetylhydrolase ELISA Kit; Platelet-activating factor acetylhydrolase ELISA Kit
缩写：PLA2G7
Uniprot No.：Q13093
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:10 | Average % | 91 | ||||
| Range % | 87-96 | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 93-100 | |||||
| 1:40 | Average % | 89 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of human Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.7672.8612.8142.642 502.3562.3132.3352.163 251.6721.6381.6551.483 12.50.9830.9720.9780.806 6.250.6570.6350.6460.474 3.120.4550.4440.4500.278 1.560.2790.2860.2830.111 00.1700.1730.172 | ||||||
货期：3-5 working days
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