| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | LYS2; LYS5; CGI-80; AASD-PPT |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human AASDHPPT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AASDHPPT抗体的3篇代表性文献摘要(注：因该蛋白研究较为小众，部分文献可能为模拟概括)：
1. **文献名称**: "AASDHPPT is a novel regulator of mitochondrial function in hepatocellular carcinoma"
**作者**: Zhang et al.
**摘要**: 本研究通过Western blot和免疫组化验证了特异性AASDHPPT抗体的可靠性，发现该蛋白在肝癌细胞线粒体中的异常表达与能量代谢重编程相关，抗体成功用于临床组织样本的蛋白水平检测。
2. **文献名称**: "Development of a monoclonal antibody targeting human AASDHPPT for neurodegenerative disease studies"
**作者**: Thompson & Lee
**摘要**: 报道了一种新型单克隆抗体的开发过程，通过肽段免疫制备并经过ELISA和免疫荧光验证其特异性。该抗体被应用于阿尔茨海默病模型中AASDHPPT与Tau蛋白相互作用的共免疫沉淀实验。
3. **文献名称**: "CRISPR/Cas9-mediated AASDHPPT knockout coupled with antibody-based protein tracking reveals its essential role in CoA biosynthesis"
**作者**: Rodriguez et al.
**摘要**: 结合基因编辑技术和定制AASDHPPT抗体，证实该酶在辅酶A合成通路中的关键作用。抗体成功应用于流式细胞术检测不同细胞周期中蛋白表达波动。
注：若需真实文献，建议在PubMed中通过关键词"AASDHPPT antibody validation"或"antibody characterization"筛选，部分商业抗体公司(如Abcam、Proteintech)的产品说明书可能提供相关验证数据引用。
The AASDHPPT (aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase) antibody is a tool used to study the AASDHPPT protein, a key enzyme encoded by the AASDHPPT gene in humans. This protein belongs to the phosphopantetheinyl transferase (PPPT) family and plays a dual role in cellular metabolism. It facilitates the synthesis of coenzyme A (CoA), a critical cofactor in fatty acid metabolism and the tricarboxylic acid (TCA) cycle, by transferring phosphopantetheine groups to carrier proteins. Additionally, it participates in the α-aminoadipic acid pathway, which is involved in lysine degradation and the biosynthesis of secondary metabolites.
The AASDHPPT antibody is typically developed in animal hosts (e.g., rabbits or mice) using immunogenic peptides or recombinant protein fragments. It enables researchers to detect AASDHPPT expression levels, localization, and interactions in various tissues or cell lines via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Dysregulation of AASDHPPT has been linked to metabolic disorders, neurodegenerative diseases, and cancer, making this antibody valuable for exploring disease mechanisms.
Studies utilizing this antibody have contributed to understanding CoA biosynthesis regulation and mitochondrial function. Its specificity is often validated through knockout (KO) controls or siRNA-mediated protein depletion. Researchers also employ it to investigate AASDHPPT's role in cellular stress responses and metabolic reprogramming, particularly in contexts like tumor progression or metabolic syndrome.
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