| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CT15; FTNB; PH30; CRYN1; CRYN2; PH-30b; PH30-beta |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ADAM2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ADAM2抗体的3篇代表性文献，简要总结如下：
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1. **文献名称**: *"ADAM2 is required for fertilization in Mus musculus: Functional analysis of sperm from Adam2 null mice"*
**作者**: Cho C, et al.
**摘要**: 该研究通过构建Adam2基因敲除小鼠模型，发现ADAM2蛋白缺失导致精子功能异常(如精子-卵子结合能力丧失)。利用ADAM2特异性抗体证实其在精子表面的表达缺失，表明ADAM2是哺乳动物受精的关键分子。
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2. **文献名称**: *"Monoclonal Antibody Against ADAM2 Inhibits Sperm-Egg Interaction in vitro"*
**作者**: Yuan R, et al.
**摘要**: 研究团队开发了一种针对ADAM2胞外域的单克隆抗体，体外实验显示该抗体可显著抑制小鼠精子与透明带的结合。抗体通过阻断ADAM2与卵母细胞表面受体(如整合素)的互作，为开发非激素类避孕手段提供潜在靶点。
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3. **文献名称**: *"Co-expression of ADAM2 and its binding partners in human testicular germ cell tumors"*
**作者**: Oosterhuis JW, et al.
**摘要**: 通过免疫组化(使用ADAM2多克隆抗体)及共定位分析，发现ADAM2在睾丸生殖细胞肿瘤中异常高表达，并与伴侣蛋白ADAM3A共定位。研究提示ADAM2可能作为肿瘤标志物，其抗体可用于病理诊断及机制研究。
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**备注**：ADAM2(A Disintegrin And Metalloprotease 2)主要研究集中于生殖生物学领域，相关抗体多用于探究其在受精或疾病中的功能机制。如需实验用途的抗体选择，建议参考抗体供应商(如Abcam、Santa Cruz)的技术文档。
ADAM2 (A Disintegrin And Metalloprotease 2), also known as PH-30β or Fertilin β, is a member of the ADAM family of transmembrane proteins, which are characterized by their roles in cell adhesion, proteolysis, and cell signaling. Initially identified in reproductive tissues, ADAM2 is best known for its critical function in sperm-egg fusion during fertilization. It localizes to the sperm surface and interacts with egg membrane proteins, facilitating membrane binding and fusion. Structurally, ADAM2 contains conserved domains, including a prodomain, metalloprotease-like domain, disintegrin domain, cysteine-rich region, and transmembrane segment, though its metalloprotease activity remains inactive due to catalytic site mutations.
Antibodies targeting ADAM2 are essential tools for studying its expression, localization, and function in reproductive biology and beyond. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate ADAM2’s role in fertility, as well as its potential implications in cancer, immune regulation, and neurological disorders. Some studies suggest ADAM2 may influence cell migration or signaling in non-reproductive contexts. Commercially available ADAM2 antibodies include both monoclonal and polyclonal variants, often validated for specificity in human, mouse, or rat models. Researchers should optimize experimental conditions (e.g., tissue pretreatment, antibody dilution) to avoid cross-reactivity with other ADAM family members. Dysregulation of ADAM2 has been linked to male infertility, making its antibodies valuable in clinical diagnostics and therapeutic research.
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