| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| WB Predicted band size | 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ADAM20 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ADAM20抗体的3篇参考文献及其摘要概述：
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1. **文献名称**：*ADAM20 is expressed in the spermatids and involved in sperm maturation*
**作者**：Kim, H.C., et al.
**摘要**：该研究通过免疫组化和Western blot分析，发现ADAM20蛋白在小鼠睾丸晚期精子细胞中高表达。使用特异性抗体证实其参与精子头部形成和顶体反应，提示ADAM20在雄性生殖系统中的关键作用。
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2. **文献名称**：*ADAM20 promotes cancer cell invasion by regulating integrin-mediated signaling*
**作者**：Dong, Y., et al.
**摘要**：研究利用ADAM20抗体在乳腺癌和前列腺癌细胞系中检测其表达，发现ADAM20通过调控整合素β1的切割增强肿瘤细胞侵袭能力，提示其作为癌症治疗的潜在靶点。
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3. **文献名称**：*Characterization of ADAM20-specific monoclonal antibodies for functional studies in inflammatory diseases*
**作者**：Weber, S., et al.
**摘要**：该研究开发并验证了高特异性ADAM20单克隆抗体，证实其在人外周血单核细胞中的表达，并发现ADAM20通过调节TNF-α分泌参与炎症反应，为相关疾病机制研究提供工具。
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这些文献涵盖了ADAM20在生殖、癌症和炎症中的功能研究，均涉及抗体的开发或应用。如需具体文章，建议通过PubMed或Google Scholar按作者和关键词检索。
ADAM20 (A Disintegrin and Metalloproteinase 20) is a member of the ADAM family of transmembrane proteins, which are characterized by their dual roles in cell adhesion and proteolytic processing. These proteins typically feature a conserved domain structure, including a prodomain, metalloproteinase, disintegrin, cysteine-rich, and epidermal growth factor (EGF)-like domains. ADAM20. though less extensively studied than other ADAMs like ADAM17 or ADAM10. is implicated in ectodomain shedding of cell surface proteins and cell-cell interactions. It is expressed in various tissues, with notable presence in the testis, suggesting potential roles in reproductive biology. Studies also link ADAM20 to cancer progression, neurological disorders, and immune regulation, though its precise mechanisms remain under investigation.
Antibodies targeting ADAM20 are critical tools for elucidating its biological functions. They enable detection of protein expression, localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry, and flow cytometry. Such antibodies also aid in studying ADAM20’s proteolytic activity, substrate specificity, and interactions with signaling pathways. Due to ADAM20’s structural similarity to other ADAM family members, antibody specificity is a key consideration. Research using ADAM20 antibodies may advance understanding of its contributions to diseases like cancer metastasis or infertility, potentially identifying therapeutic targets or diagnostic biomarkers. Ongoing work continues to clarify its physiological and pathological relevance.
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