| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | DCAF3; WDR94 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human AMBRA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AMBRA1抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *AMBRA1 links autophagy to cell proliferation and tumorigenesis by regulating c-Myc and mTOR*
**作者**: Cianfanelli V, et al.
**摘要**: 该研究揭示AMBRA1通过调控c-Myc蛋白稳定性和mTOR信号通路，平衡细胞自噬与增殖，其缺失导致小鼠胚胎发育异常和肿瘤易感性增加。实验中使用AMBRA1抗体验证了其在自噬体形成中的关键作用。
2. **文献名称**: *Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation*
**作者**: Nazio F, et al.
**摘要**: 研究发现AMBRA1通过结合ULK1复合体动态调节自噬启动，使用特异性抗体证实其与Beclin-1的相互作用，并揭示其在细胞周期依赖的自噬振荡中的调控机制。
3. **文献名称**: *The dynamic interaction of AMBRA1 with the dynein motor complex regulates mammalian autophagy*
**作者**: Di Bartolomeo S, et al.
**摘要**: 该论文证明AMBRA1通过与动力蛋白复合物结合，介导自噬前体细胞器的运输。利用AMBRA1抗体进行免疫沉淀实验，揭示了其在营养缺乏条件下启动自噬的空间调控机制。
这些研究均通过AMBRA1抗体在Western blot、免疫共沉淀或免疫荧光等实验中，阐明了该蛋白在自噬、癌症及细胞周期中的多功能调控机制。
**Background of AMBRA1 Antibody**
AMBRA1 (Activating Molecule in Bechin-1-Regulated Autophagy) is a crucial regulatory protein involved in autophagy, a cellular degradation process essential for maintaining homeostasis. It interacts with the Beclin-1 complex to promote autophagosome formation and regulate autophagic flux. AMBRA1 also plays roles beyond autophagy, including cell proliferation, apoptosis, and neurodevelopment, by modulating signaling pathways like mTOR and ULK1. Dysregulation of AMBRA1 has been linked to cancer, neurodegenerative diseases, and developmental disorders.
AMBRA1 antibodies are tools used to detect and study the expression, localization, and function of AMBRA1 in various biological contexts. These antibodies are widely applied in techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Researchers utilize them to investigate AMBRA1’s involvement in autophagy-related mechanisms, its interaction partners, and its pathological implications. High-quality AMBRA1 antibodies are characterized by specificity, sensitivity, and reproducibility, enabling insights into its dual roles as a tumor suppressor (via autophagy induction) and oncogene (in specific contexts). Their development and validation have significantly advanced understanding of autophagy regulation and its therapeutic targeting in disease.
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