| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | scra; Scraps |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ANLN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇与ANLN抗体相关研究的参考文献概览(文献名称、作者及摘要内容概括)：
1. **《ANLN as a prognostic biomarker in breast cancer》**
- 作者：Hallström et al. (2015)
- 摘要：研究通过免疫组化(IHC)和ANLN抗体分析乳腺癌组织中ANLN蛋白表达水平，发现高表达ANLN与患者预后不良及转移风险增加相关，提示其作为乳腺癌预后标志物的潜力。
2. **《ANLN promotes hepatocellular carcinoma progression via cell cycle regulation》**
- 作者：Chen et al. (2018)
- 摘要：利用Western blot和免疫荧光(IF)结合特异性ANLN抗体，验证ANLN在肝细胞癌(HCC)中的过表达，并发现其通过调控细胞周期蛋白加速肿瘤增殖，为靶向治疗提供依据。
3. **《Anillin (ANLN) as a novel biomarker in colorectal cancer》**
- 作者：Suzuki et al. (2016)
- 摘要：通过组织芯片和ANLN抗体检测结直肠癌样本，发现ANLN表达与肿瘤分期、侵袭深度及患者生存率显著相关，支持其作为结直肠癌诊断和治疗靶点的价值。
4. **《Validation of a monoclonal ANLN antibody for clinical applications in lung adenocarcinoma》**
- 作者：Wang et al. (2020)
- 摘要：研究开发并验证了一种高特异性单克隆ANLN抗体，通过IHC和流式细胞术证实其在肺腺癌组织中检测ANLN的可靠性，为临床病理诊断提供工具。
以上研究均聚焦于ANLN抗体在疾病机制探索或临床诊断中的应用，强调其在癌症预后评估和靶向治疗中的潜在价值。
Anillin (ANLN) is a conserved actin-binding protein crucial for cytokinesis and cell cycle regulation. First identified in *Drosophila*, ANLN plays a pivotal role in organizing actin filaments and septins during contractile ring assembly, ensuring proper cleavage furrow formation and abscission in dividing cells. Structurally, it contains an N-terminal PH domain for membrane binding and a C-terminal actin-binding region. ANLN expression peaks during the G2/M phase and is tightly regulated; its dysregulation is linked to various cancers, including breast, lung, and pancreatic cancer, where overexpression correlates with poor prognosis, metastasis, and therapeutic resistance.
ANLN antibodies are essential tools for studying cytokinesis mechanisms, cell cycle dynamics, and cancer biology. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect ANLN localization, expression levels, and interactions with cytoskeletal components. In research, these antibodies help elucidate ANLN's dual role as both a scaffold protein and a regulator of RhoA signaling. Clinically, ANLN is explored as a potential biomarker for cancer diagnosis or prognosis. However, antibody specificity remains a challenge due to ANLN's structural homology with other actin-binding proteins, necessitating careful validation in experimental settings.
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