| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ACY-1; ACY1D |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ACY1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ACY1抗体的参考文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库查询确认)：
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1. **标题**: "Immunohistochemical Analysis of ACY1 Expression in Human Renal Tubular Cells"
**作者**: Smith J, et al.
**摘要**: 本研究通过免疫组化技术检测ACY1在健康与慢性肾病患者的肾小管细胞中的表达差异，发现ACY1蛋白水平在代谢紊乱患者中显著降低，提示其可能作为肾功能异常的潜在生物标志物。
2. **标题**: "Development of a Novel Anti-ACY1 Monoclonal Antibody for Metabolic Disorder Diagnosis"
**作者**: Tanaka K, et al.
**摘要**: 文章报道了一种高特异性抗人ACY1单克隆抗体的开发，并验证其在ELISA和Western blot中的应用。该抗体成功用于检测遗传性ACY1缺陷症患者的血清样本，为临床诊断提供了新工具。
3. **标题**: "ACY1 Protein Localization and Its Role in Neurological Disorders"
**作者**: Müller R, et al.
**摘要**: 通过免疫荧光和免疫印迹技术，研究发现ACY1在小鼠大脑神经元中高表达，且在阿尔茨海默病模型中表达异常，提示ACY1可能与神经退行性病变相关。
4. **标题**: "Comparative Study of ACY1 Antibody Performance in Cancer Biomarker Screening"
**作者**: Chen L, et al.
**摘要**: 对比多种商用ACY1抗体的灵敏度和特异性，发现抗体Clone X-203在肝癌组织中的检测效果最优，为ACY1作为肿瘤标志物的研究提供了方法学参考。
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建议通过PubMed或Google Scholar搜索关键词“ACY1 antibody”、“Aminoacylase 1”、“ACY1 immunohistochemistry”以获取真实文献。
ACY1 (Aminoacylase-1) is a zinc-binding enzyme belonging to the M20 peptidase family, primarily involved in catalyzing the hydrolysis of N-acylated amino acids into free amino acids and acetic acid derivatives. It plays a critical role in cellular nitrogen metabolism and the degradation of acetylated proteins. The ACY1 gene is located on chromosome 3p21.1. and its deficiency is linked to a rare metabolic disorder called aminoacylase 1 deficiency, characterized by urinary excretion of specific N-acetylated amino acids and neurological abnormalities.
ACY1 antibodies are immunological tools developed to detect and quantify ACY1 protein expression in biological samples. These antibodies are widely used in research to study ACY1's tissue distribution, subcellular localization (primarily cytosolic), and regulatory mechanisms. They are also employed in diagnostic settings to investigate metabolic disorders or validate ACY1 downregulation in certain cancers, such as renal cell carcinoma. Commercially available ACY1 antibodies include polyclonal and monoclonal variants, often validated for applications like Western blotting, immunohistochemistry, and ELISA. Specificity and cross-reactivity are critical considerations, with rigorous validation required to ensure minimal off-target binding. Recent studies also explore ACY1's potential as a biomarker in neurodegenerative diseases, expanding the utility of these antibodies in both basic and clinical research contexts.
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