| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human AUP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于AUP1抗体的3篇参考文献示例(内容基于典型研究方向，非真实文献)：
1. **标题**: "AUP1 regulates hepatic lipid metabolism via ubiquitination pathways"
**作者**: Smith J, et al.
**摘要**: 研究利用AUP1抗体通过Western blot和免疫组化发现，AUP1通过泛素化修饰调控肝脏脂滴形成，缺失AUP1会加剧脂肪变性。
2. **标题**: "AUP1 mediates ER-associated degradation through interaction with HRD1 complex"
**作者**: Tanaka R, et al.
**摘要**: 通过AUP1抗体的免疫共沉淀实验，揭示AUP1与内质网降解复合体HRD1的结合，促进错误折叠蛋白的泛素化降解。
3. **标题**: "Viral exploitation of AUP1 in host lipid remodeling"
**作者**: Chen L, et al.
**摘要**: 使用AUP1抗体的免疫荧光技术，证明某些病毒劫持宿主AUP1蛋白，改变脂膜结构以支持病毒复制，抗体阻断实验进一步验证其功能。
4. **标题**: "AUP1 overexpression correlates with poor prognosis in colorectal cancer"
**作者**: Wang Y, et al.
**摘要**: 通过AUP1抗体的组织芯片分析，发现结直肠癌中AUP1高表达与转移相关，提示其作为潜在治疗靶点。
---
注：以上文献为示例，实际引用时需检索真实数据库(如PubMed)并核对原文。
The AUP1 (Ancient Ubiquitous Protein 1) antibody is a tool used to study the AUP1 protein, a conserved eukaryotic protein involved in diverse cellular processes. AUP1 contains an N-terminal AID (AUP1-specific lipid-binding domain), a central ubiquitin-like (UBL) domain, and a C-terminal G2BR (Graf2-binding region), enabling interactions with lipids, ubiquitination machinery, and other proteins. It localizes to the endoplasmic reticulum (ER) and lipid droplets (LDs), playing roles in ER-associated degradation (ERAD), lipid storage, and autophagosome formation. AUP1 bridges autophagy and lipid metabolism by recruiting core autophagy proteins (e.g., ATG8) to LDs and facilitating LD degradation via lipophagy. Dysregulation of AUP1 is linked to metabolic disorders, neurodegenerative diseases, and cancer progression.
The AUP1 antibody is widely used in research to detect AUP1 expression and study its functions. Applications include Western blotting, immunofluorescence, and immunohistochemistry to analyze protein levels, subcellular localization, and interactions in various tissues or cell lines. Commercial AUP1 antibodies are typically validated for specificity against human, mouse, or rat homologs. Studies utilizing this antibody have contributed to understanding AUP1's role in cellular stress responses, lipid homeostasis, and disease mechanisms. However, researchers should verify antibody performance in specific experimental contexts due to potential cross-reactivity or batch variability.
×
