| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HPP1; ula-1; APPBP1; A-116A10.1 |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NAE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NAE1抗体的3篇参考文献及其摘要内容的简要整理：
1. **文献名称**：*Inhibition of NEDD8-activating enzyme induces rereplication and apoptosis in human tumor cells*
**作者**：Soucy, T. A., et al.
**摘要**：该研究揭示了NAE1(NEDD8激活酶)在肿瘤细胞中的关键作用，通过使用小分子抑制剂MLN4924阻断其活性，导致细胞周期蛋白的异常积累和DNA损伤，从而引发肿瘤细胞凋亡。研究中通过NAE1抗体验证了抑制剂对NAE1蛋白表达的调控作用。
2. **文献名称**：*MLN4924. a NEDD8-activating enzyme inhibitor, blocks protein neddylation and induces autophagy in cancer cells*
**作者**：Milhollen, M. A., et al.
**摘要**：本文探讨了NAE1抑制剂MLN4924通过抑制蛋白质的neddylation修饰，激活自噬通路并抑制肿瘤细胞生长的机制。研究中利用NAE1抗体进行Western blot分析，证实了NAE1在多种癌细胞系中的表达及其功能抑制后的分子变化。
3. **文献名称**：*NAE1 overexpression correlates with poor prognosis and promotes tumor progression in colorectal cancer*
**作者**：Zhang, Y., et al.
**摘要**：该研究通过免疫组化(使用NAE1特异性抗体)分析了结直肠癌组织中NAE1的表达水平，发现其高表达与患者预后不良相关。进一步实验表明，NAE1通过调控细胞周期和凋亡相关蛋白促进肿瘤进展，为NAE1作为治疗靶点提供了依据。
这些文献均涉及NAE1抗体的应用(如蛋白表达检测、功能机制研究)，并围绕其在癌症治疗中的潜在价值展开。
The NAE1 antibody targets the NEDD8-activating enzyme E1 subunit 1 (NAE1), a critical component of the NEDDylation pathway. NEDDylation is a post-translational modification process analogous to ubiquitination, where the ubiquitin-like protein NEDD8 is conjugated to specific substrate proteins, primarily cullin family members. This modification regulates the activity of cullin-RING E3 ubiquitin ligases (CRLs), which control the degradation of proteins involved in cell cycle progression, DNA repair, and signal transduction. NAE1. along with its partner UBA3. forms the heterodimeric NEDD8-activating enzyme responsible for NEDD8 activation and transfer to target proteins. Dysregulation of NAE1 and the NEDDylation pathway has been implicated in various cancers, as aberrant CRL activity can lead to uncontrolled cell proliferation and tumorigenesis.
Antibodies against NAE1 are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry, and co-immunoprecipitation to investigate NAE1's role in cancer, neurodegenerative diseases, and immune responses. Additionally, NAE1 has emerged as a therapeutic target, with inhibitors like MLN4924 (Pevonedistat) being developed to block NEDDylation in malignancies. Research using NAE1 antibodies has provided insights into disease mechanisms and potential biomarkers, highlighting their significance in translational and clinical studies.
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