| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | C1orf169 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human APOBEC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于APOBEC4抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: "APOBEC4 Enhances the Replication of HIV-1 through RNA Editing"
**作者**: Koito, M., & Ikeda, T.
**摘要**: 该研究探讨了APOBEC4在HIV-1复制中的作用，通过开发特异性抗体验证其RNA编辑活性。实验表明APOBEC4可能通过脱氨基作用调控病毒RNA，抗体用于检测其在细胞中的表达及定位。
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2. **文献名称**: "Characterization of APOBEC4 Antibody for Immunohistochemical Analysis in Germ Cells"
**作者**: Zhang, J., et al.
**摘要**: 研究团队制备了针对人APOBEC4的多克隆抗体，并验证其在Western blot和免疫组化中的特异性。结果显示APOBEC4在睾丸生殖细胞中高表达，提示其可能在生殖发育中发挥作用。
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3. **文献名称**: "Cross-Reactivity of APOBEC Family Antibodies: A Comparative Study"
**作者**: Sánchez-Martínez, A., et al.
**摘要**: 本文系统比较了商业及实验室自制的APOBEC家族抗体(包括APOBEC4)的特异性，发现部分抗体存在与APOBEC3成员的交叉反应，强调了验证抗体特异性的重要性。
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**备注**：APOBEC4的研究相对较少，上述文献为虚构示例，实际研究中可能需要查阅近年来的生化学或病毒学期刊数据库(如PubMed)。建议通过关键词“APOBEC4 antibody validation”或“APOBEC4 immunological tools”检索最新成果。
APOBEC4 (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4) is a member of the APOBEC family of cytidine deaminases, which are broadly involved in RNA or DNA editing processes. While other APOBEC proteins, such as APOBEC3 and AID (activation-induced deaminase), are well-characterized for their roles in antiviral defense, antibody diversification, and genome stability, APOBEC4 remains less understood. It shares structural features with the family, including conserved zinc-coordinating motifs critical for catalytic activity, but its biological functions and substrates are still under investigation.
Initial studies suggest APOBEC4 may contribute to DNA repair or recombination pathways, potentially interacting with genomic DNA or RNA in germ cells or somatic tissues. Unlike APOBEC3 enzymes, which restrict retroelements and viruses like HIV, APOBEC4 shows no strong antiviral activity in current models. Its expression appears restricted, with low levels detected in the testis and certain cancers, hinting at tissue-specific roles. However, conflicting reports and limited functional data necessitate further validation.
Antibodies targeting APOBEC4 have been developed primarily as research tools to study its expression patterns, subcellular localization, and interactions. These reagents enable detection via Western blot, immunohistochemistry, or immunofluorescence, aiding in mapping its tissue distribution and potential involvement in diseases like cancer or infertility. Challenges persist in distinguishing APOBEC4 from homologous family members due to sequence similarities, emphasizing the need for highly specific antibodies. Ongoing research aims to clarify APOBEC4's physiological significance, catalytic targets, and pathological implications, which could unlock therapeutic or diagnostic applications.
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