| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | APC5 |
| WB Predicted band size | 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ANAPC5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ANAPC5抗体的参考文献示例，内容基于研究领域常见主题，但文献标题和作者为模拟概括，建议通过学术数据库进一步验证：
1. **"Structural Insights into the Human Anaphase-Promoting Complex Using Cryo-EM"**
*Authors: Zhang, Y., Barford, D.*
**摘要**：通过冷冻电镜解析APC/C复合体的高分辨率结构，研究ANAPC5亚基的空间定位及功能，使用特异性抗体验证其与其他亚基的相互作用及复合体组装机制。
2. **"ANAPC5 Knockdown Disrupts Mitotic Exit and Chromosome Segregation"**
*Authors: Peters, J.M., Meyer-Schaller, S.*
**摘要**：探讨ANAPC5在细胞周期退出中的关键作用，通过RNA干扰和ANAPC5抗体进行Western blot及免疫荧光实验，证实其缺失导致染色体分离异常。
3. **"ANAPC5 Overexpression Correlates with Poor Prognosis in Colorectal Cancer"**
*Authors: Wang, Y., Li, X.*
**摘要**：分析ANAPC5在结直肠癌中的表达水平，利用免疫组化(使用ANAPC5抗体)发现其高表达与肿瘤进展和患者生存率降低显著相关。
4. **"APC/C Subunit Interaction Networks Revealed by Co-Immunoprecipitation"**
*Authors: Zhou, H., da Fonseca, A.*
**摘要**：通过共免疫沉淀技术(结合ANAPC5抗体)研究APC/C亚基间的动态互作，揭示其在泛素化底物识别中的调控机制。
**建议**：如需具体文献，可通过PubMed或Google Scholar搜索关键词“ANAPC5 antibody”或“APC5 antibody”，筛选实验应用或疾病相关研究。
The Anaphase-Promoting Complex/Cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase essential for cell cycle regulation, particularly during the transition from metaphase to anaphase and exit from mitosis. ANAPC5 (APC5) is one of the core components of the APC/C complex, contributing to its structural integrity and substrate recognition. It plays a critical role in mediating the ubiquitination of key cell cycle regulators, such as securin and cyclin B, targeting them for proteasomal degradation. This process ensures proper chromosome segregation and mitotic progression.
ANAPC5-specific antibodies are widely used in research to study APC/C assembly, function, and localization. They enable the detection of ANAPC5 expression levels in various cell types or tissues, aiding investigations into cell cycle dysregulation linked to diseases like cancer, neurodegeneration, or developmental disorders. For example, reduced ANAPC5 expression has been associated with mitotic defects and genomic instability. These antibodies are also employed in co-immunoprecipitation assays to explore interactions between ANAPC5 and other APC/C subunits or regulatory cofactors (e.g., CDC20 or CDH1).
As a conserved protein across eukaryotes, ANAPC5's functional studies in model organisms further highlight its evolutionary importance. Research using ANAPC5 antibodies continues to unravel its role beyond mitosis, including potential involvement in cellular stress responses and apoptosis.
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