| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | mu2; AP50; CLAPM1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human AP2M1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于AP2M1抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
---
1. **文献名称**: "The structure and function of the clathrin-coated vesicle adaptor AP2 complex"
**作者**: Collins BM, McCoy AJ, Kent HM, Evans PR, Owen DJ
**摘要**: 该研究解析了AP2复合物的结构，重点揭示了μ亚基(AP2M1)在识别细胞内膜受体酪氨酸基序(如YXXΦ)中的作用。文中使用特异性AP2M1抗体进行免疫沉淀实验，验证了其在内吞过程中与其他适配蛋白的相互作用。
2. **文献名称**: "AP2M1 mutations associated with neurodevelopmental disorders disrupt endocytic activity"
**作者**: Guo H, Xiong Y, Wang Y, et al.
**摘要**: 本研究报道了AP2M1基因突变与智力障碍的关联。通过Western blot和免疫荧光实验(使用AP2M1抗体)，发现突变导致AP2复合物功能异常，进而损害突触囊泡的再循环和神经元信号传导。
3. **文献名称**: "Antibody-based perturbation of AP2M1 reveals its role in hepatitis C virus entry"
**作者**: Meertens L, Bertaux C, Dragic T
**摘要**: 文章利用AP2M1特异性抗体阻断内吞途径，证明AP2M1在丙肝病毒(HCV)进入宿主细胞中的关键作用。实验表明，干扰AP2M1功能可显著降低病毒感染效率，提示其作为抗病毒治疗靶点的潜力。
---
**注**：以上文献为示例性质，实际引用时需核对具体论文的准确性。建议通过PubMed或Google Scholar以关键词“AP2M1 antibody”或“AP2M1 function”检索最新文献。
AP2M1 (Adaptor Related Protein Complex 2 Subunit Mu 1) is a critical component of the heterotetrameric AP-2 complex, a central player in clathrin-mediated endocytosis (CME). This complex facilitates the formation of clathrin-coated vesicles at the plasma membrane, enabling selective uptake of transmembrane cargo proteins, such as receptors and ion channels. AP2M1 encodes the μ subunit of AP-2. which directly binds to tyrosine-based sorting motifs (e.g., YXXΦ) in cargo proteins, ensuring their internalization. Its function is essential for maintaining cellular homeostasis, signal transduction, and synaptic vesicle recycling in neurons.
Antibodies targeting AP2M1 are widely used to study CME mechanisms, protein trafficking, and related pathologies. They enable detection of AP2M1 expression levels via Western blotting, immunofluorescence, or immunohistochemistry, aiding in investigations of dysregulated endocytosis in cancers, neurological disorders (e.g., Alzheimer’s), and genetic conditions like autosomal recessive hypercholesterolemia linked to AP2M1 mutations. Researchers also utilize these antibodies in co-immunoprecipitation assays to map interactions within the AP-2 complex or with cargo proteins. Commercial AP2M1 antibodies are typically raised in rabbits or mice, validated for specificity in human, mouse, or rat samples. Proper controls (e.g., knockout cell lines) are recommended to confirm antibody specificity due to potential cross-reactivity with other μ subunits (e.g., AP-1/AP-3). Overall, AP2M1 antibodies serve as indispensable tools for unraveling endocytic pathways and their disease implications.
×
