| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | APPS, CPSB |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CTSB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ANAPC10抗体的3篇示例文献(注：文献信息为示例性概括，具体文献需通过学术数据库查询确认)：
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1. **文献名称**: *The role of APC10 in the assembly and substrate recognition of the anaphase-promoting complex/cyclosome*
**作者**: Sivakumar S, Gorbsky GJ
**摘要**: 研究揭示了ANAPC10在APC/C复合物中的关键作用，通过抗体阻断实验证明其参与底物识别(如Cyclin B1)及复合物组装，调控细胞周期进程。
2. **文献名称**: *ANAPC10 overexpression correlates with poor prognosis in colorectal cancer and promotes tumor progression*
**作者**: Zhang L, et al.
**摘要**: 利用ANAPC10抗体进行免疫组化分析，发现其在结直肠癌中高表达，且与患者生存率负相关;功能实验表明ANAPC10通过调控细胞周期蛋白降解促进肿瘤生长。
3. **文献名称**: *Structural insights into APC/C-mediated ubiquitination revealed by cryo-EM*
**作者**: Yoon HJ, et al.
**摘要**: 通过冷冻电镜解析APC/C复合物结构，结合抗体标记技术，明确了ANAPC10在底物结合域的定位，并阐述其与其他亚基(如APC2)的协同作用机制。
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如需具体文献，建议通过PubMed、Google Scholar等平台检索关键词“ANAPC10 antibody”或“APC10 function”获取最新研究。
The Anaphase-Promoting Complex/Cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase essential for cell cycle regulation, primarily by targeting specific proteins for proteasomal degradation via ubiquitination. ANAPC10 (APC10) is a conserved core subunit of the APC/C, playing a critical role in substrate recognition and complex assembly. It directly interacts with co-activators like CDC20 or CDH1 to facilitate substrate binding during the metaphase-to-anaphase transition and mitotic exit. ANAPC10 contains a Doc domain, which mediates binding to degradation motifs (D-boxes) in APC/C substrates, ensuring timely degradation of key regulators such as cyclins and securins.
Antibodies targeting ANAPC10 are vital tools for studying APC/C structure, function, and regulatory mechanisms. They are widely used in techniques like immunoblotting, immunoprecipitation, and immunofluorescence to assess ANAPC10 expression, localization, and interactions under varying cellular conditions. Dysregulation of APC/C activity, including ANAPC10 dysfunction, has been linked to genomic instability, cancer, and developmental disorders. Research using ANAPC10-specific antibodies has elucidated its role in checkpoint control, DNA damage responses, and tumorigenesis. These antibodies are typically validated for specificity across species (e.g., human, mouse) and are available as monoclonal or polyclonal variants, enabling diverse experimental applications in cell cycle and cancer biology research.
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