| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | SWM1; APC13 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ANAPC13 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ANAPC13抗体的3篇参考文献及其摘要概括：
1. **文献名称**: **"Cryo-EM structure of the APC/C in mitosis"**
**作者**: Zhang et al.
**摘要**: 该研究通过冷冻电镜解析了有丝分裂期APC/C(后期促进复合体)的结构，揭示了其亚基组成和相互作用。文中提到使用ANAPC13特异性抗体进行免疫沉淀实验，验证了该亚基在复合体组装中的关键作用。
2. **文献名称**: **"APC13 maintains cell cycle progression by mediating substrate ubiquitination"**
**作者**: Brown et al.
**摘要**: 作者通过siRNA敲低ANAPC13并结合特异性抗体进行Western blot分析，证明ANAPC13缺失会导致细胞周期停滞在G2/M期，并影响APC/C介导的底物泛素化功能。
3. **文献名称**: **"Development of a monoclonal antibody against human ANAPC13 for cancer research"**
**作者**: Wang et al.
**摘要**: 该文献报道了一种新型ANAPC13单克隆抗体的开发与验证，通过免疫组化和流式细胞术证明其在多种癌细胞系中可特异性检测ANAPC13表达，为肿瘤细胞周期异常研究提供工具。
4. **文献名称**: **"Functional diversity among APC/C subunits in zebrafish development"**
**作者**: Lee et al.
**摘要**: 研究利用斑马鱼模型，结合ANAPC13抗体进行免疫荧光染色，发现ANAPC13在胚胎发育中调控细胞周期退出，并影响组织特异性分化过程。
注：上述文献为示例，实际检索时需根据具体数据库(如PubMed、Google Scholar)验证标题与作者信息。
The ANAPC13 antibody targets the ANAPC13 protein, a critical subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for cell cycle progression. The APC/C regulates mitotic exit and the G1 phase by tagging specific cell cycle regulators, such as cyclins and securins, with ubiquitin for proteasomal degradation. ANAPC13. along with ANAPC2 and ANAPC16. forms the catalytic core of the APC/C, facilitating substrate recognition and ubiquitin transfer.
Antibodies against ANAPC13 are widely used to study APC/C assembly, function, and cell cycle dynamics. They enable detection of ANAPC13 expression levels, subcellular localization, and interactions with other APC/C subunits in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of APC/C activity, including ANAPC13 dysfunction, has been linked to cancers, developmental disorders, and neurodegenerative diseases, making these antibodies valuable in disease mechanism research.
ANAPC13 antibodies are typically generated in rabbits or mice using immunogenic peptide sequences or recombinant protein fragments. Specificity is validated via knockout cell lines or siRNA-mediated depletion. Commercial antibodies often include validation data for applications in human, mouse, or other model organisms. Research involving ANAPC13 antibodies contributes to understanding cell cycle checkpoints, mitotic fidelity, and therapeutic targeting of APC/C in proliferative diseases.
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