| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | GFI2; p190-B; RhoGAP5; p190BRhoGAP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ARHGAP5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARHGAP5抗体的3篇参考文献示例(文献信息为模拟示例，非真实存在)：
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1. **文献名称**: *ARHGAP5 promotes tumor invasion by regulating RhoA activity in triple-negative breast cancer*
**作者**: Zhang L, et al.
**摘要**: 本研究利用ARHGAP5特异性抗体通过免疫组化(IHC)和Western blot分析，发现ARHGAP5在乳腺癌组织中的高表达与RhoA信号通路激活相关，促进癌细胞侵袭转移。
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2. **文献名称**: *ARHGAP5 knockdown suppresses glioblastoma progression via EMT inhibition*
**作者**: Wang Y, et al.
**摘要**: 通过ARHGAP5抗体进行免疫荧光和流式细胞术检测，证明沉默ARHGAP5可抑制胶质母细胞瘤上皮-间质转化(EMT)，降低细胞迁移能力。
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3. **文献名称**: *ARHGAP5 as a biomarker for lung adenocarcinoma prognosis*
**作者**: Tanaka K, et al.
**摘要**: 研究使用ARHGAP5抗体对肺癌组织芯片进行染色，发现ARHGAP5高表达与患者不良预后显著相关，提示其作为潜在预后标志物的价值。
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注：以上文献为示例性质，实际文献需通过PubMed、Google Scholar等平台检索确认。若需真实文献，建议使用关键词“ARHGAP5 antibody”或“ARHGAP5 biomarker”结合近年发表年份筛选。
The ARHGAP5 antibody is a research tool targeting the ARHGAP5 protein, also known as Rho GTPase-activating protein 5 or p190-B. ARHGAP5 belongs to the RhoGAP family, which regulates Rho GTPases—key signaling molecules involved in cytoskeletal reorganization, cell migration, and proliferation. Specifically, ARHGAP5 inactivates RhoA by accelerating its GTP hydrolysis, switching it from an active (GTP-bound) to an inactive (GDP-bound) state. This activity modulates cell adhesion, motility, and contractility, making ARHGAP5 critical in processes like cancer metastasis, neuronal development, and immune responses.
ARHGAP5 antibodies are widely used in studies to detect protein expression, localization, and function in cell lines, tissues, or experimental models. They are employed in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Dysregulation of ARHGAP5 has been linked to tumor progression, particularly in cancers like glioblastoma and breast cancer, where its overexpression may promote invasiveness. Conversely, reduced ARHGAP5 levels are associated with neurological disorders, highlighting its context-dependent roles.
Researchers also use ARHGAP5 antibodies to explore its interaction partners and post-translational modifications, such as phosphorylation, which fine-tune its activity. Despite its importance, ARHGAP5's dual roles (tumor-suppressive or oncogenic) in different contexts remain under investigation, necessitating specific, high-affinity antibodies for accurate detection. Validated antibodies are essential to avoid cross-reactivity with homologous proteins like ARHGAP35 (p190-A), ensuring reliable data in both basic and translational studies.
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