| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GALT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于DNMT3A抗体的参考文献摘要简述：
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1. **文献名称**：*DNMT3A in Leukemia: Co-operation with Other Genetic Lesions*
**作者**：Ley TJ et al.
**摘要**：该研究通过Western blot和免疫沉淀实验，使用DNMT3A特异性抗体，揭示了DNMT3A基因突变在急性髓系白血病(AML)中的关键作用，发现其功能异常导致DNA甲基化模式改变，促进白血病发生。
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2. **文献名称**：*Dnmt3a is essential for hematopoietic stem cell differentiation*
**作者**：Challen GA et al.
**摘要**：利用DNMT3A抗体进行免疫组化和流式细胞术分析，证明DNMT3A在小鼠造血干细胞分化中的必要性，其缺失导致干细胞自我更新异常和分化阻滞，与血液系统疾病相关。
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3. **文献名称**：*Structural basis for DNMT3A-mediated de novo DNA methylation*
**作者**：Zhang ZM et al.
**摘要**：通过X射线晶体学结合DNMT3A抗体进行蛋白纯化验证，解析了DNMT3A与DNA结合的分子机制，阐明其在从头DNA甲基化中的构象变化及催化活性调控。
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4. **文献名称**：*DNMT3A mutations in peripheral T-cell lymphoma*
**作者**：Couronné L et al.
**摘要**：研究采用免疫印迹和免疫荧光技术(基于DNMT3A抗体)，发现外周T细胞淋巴瘤患者中DNMT3A突变导致异常甲基化谱，与肿瘤侵袭性和预后不良相关。
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提示：具体实验方法(如抗体克隆号、品牌)需参考原文材料与方法部分。如需全文链接或补充信息可进一步说明。
DNMT3A (DNA methyltransferase 3A) is a critical enzyme involved in de novo DNA methylation, a key epigenetic mechanism that regulates gene expression by adding methyl groups to cytosine residues in CpG dinucleotides. It plays a vital role during embryonic development, cellular differentiation, and maintaining genomic stability. DNMT3A, along with its homolog DNMT3B, establishes methylation patterns in early development, unlike DNMT1. which primarily maintains methylation during DNA replication. Dysregulation of DNMT3A is implicated in various diseases, including cancer (e.g., acute myeloid leukemia, lymphoma) and neurodevelopmental disorders.
Antibodies targeting DNMT3A are essential tools for studying its expression, localization, and function in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to investigate DNMT3A's role in epigenetic regulation. Specific DNMT3A antibodies help identify mutations or aberrant expression linked to disease mechanisms, particularly in hematologic malignancies where DNMT3A mutations are recurrent.
Validating DNMT3A antibodies is crucial due to potential cross-reactivity with homologous proteins (e.g., DNMT3B) or isoforms. High-quality antibodies enable research into DNMT3A interactions with partner proteins (e.g., DNMT3L) or its recruitment to specific genomic regions. Recent studies also explore its non-catalytic roles in chromatin remodeling and transcriptional repression. As DNMT3A emerges as a therapeutic target, its antibodies remain indispensable for basic research and clinical biomarker development.
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