| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | TIP; ESA1; PLIP; TIP60; cPLA2; HTATIP; ZC2HC5; HTATIP1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human KAT5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KAT5抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"KAT5/TIP60 in DNA damage response and epigenetic regulation"*
**作者**：Sapountzi, V., & Côté, J. (2006)
**摘要**：该研究探讨了KAT5(TIP60)在DNA损伤修复中的作用，通过Western blot和免疫沉淀实验验证KAT5抗体特异性，发现KAT5通过乙酰化组蛋白H4调控DNA损伤应答通路。
2. **文献名称**：*"KAT5 expression is associated with poor prognosis in breast cancer"*
**作者**：Tang, Y., et al. (2013)
**摘要**：利用免疫组化(IHC)结合KAT5抗体分析乳腺癌组织样本，发现KAT5高表达与患者生存率降低相关，提示其可能作为肿瘤预后标志物。
3. **文献名称**：*"TIP60/KAT5 is required for neuronal viability through phosphorylation of tau"*
**作者**：Kim, H., et al. (2015)
**摘要**：研究通过免疫荧光和免疫印迹(使用KAT5抗体)证明，KAT5通过调节tau蛋白磷酸化影响神经元存活，与阿尔茨海默病病理机制相关。
4. **文献名称**：*"KAT5-mediated acetylation orchestrates genomic stability and pluripotency"*
**作者**：Lee, J. E., et al. (2018)
**摘要**：结合ChIP-seq和KAT5抗体染色，揭示KAT5通过乙酰化特定基因启动子维持干细胞多能性，并参与基因组稳定性调控。
这些研究均利用KAT5抗体进行功能验证，涵盖癌症、神经疾病及干细胞领域，展示了KAT5在表观遗传调控中的关键作用。
The KAT5 antibody is a crucial tool in studying the KAT5 protein, a histone acetyltransferase (HAT) also known as TIP60. Belonging to the MYST family of HATs, KAT5 plays a pivotal role in chromatin remodeling by acetylating histones, particularly H4 and H2A, thereby regulating gene expression, DNA repair, and cellular stress responses. It is integral to multiple pathways, including the DNA damage response (DDR) via interaction with ATM/ATR kinases, apoptosis regulation through acetylation of p53. and cell cycle control. Dysregulation of KAT5 has been linked to cancers, neurodegenerative diseases, and aging.
KAT5 antibodies are widely used in research to detect and quantify the protein’s expression, localization, and activity in cells or tissues. Applications include Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP-seq). Specific antibodies may target distinct epitopes or post-translational modifications, aiding in functional studies of KAT5’s role in transcriptional regulation or DDR. Commercial KAT5 antibodies vary in host species, clonality, and validation standards, requiring careful selection for experimental reproducibility. Recent studies also explore KAT5 as a therapeutic target, with antibodies facilitating drug discovery by monitoring inhibition or activation effects. Rigorous validation remains essential due to cross-reactivity risks with related HATs or nonspecific binding.
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