首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠E选择素(E-Selectin/CD62E)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SELE含量。SELE（选择素E）是一种细胞表面糖蛋白，属于选择素家族，主要在血管内皮细胞上表达。它在炎症反应中起到关键作用，通过介导白细胞与内皮细胞之间的粘附，促进白细胞在炎症部位的聚集。SELE的研究涉及其在炎症、肿瘤和免疫调节等过程中的作用机制。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。试剂盒适用于体外检测小鼠模型中炎症相关疾病、免疫调控机制或药物干预效果的评估，例如通过检测感染模型、自身免疫疾病模型或肿瘤微环境中的组织样本，分析内皮细胞激活状态及炎症程度。适用于分子生物学、免疫学及病理机制研究等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
缩写：SELE
Uniprot No.：Q00690
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse E-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 90-96 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 81-90 | ||||||
| 1:4 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
| 1:8 | Average % | 86 | |||||
| Range % | 82-90 | ||||||
回收率：
| The recovery of mouse E-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 98-106 | |||||
| EDTA plasma (n=4) | 89 | 85-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 50002.0762.1312.1041.907 25001.6711.6381.6551.458 12501.1681.2011.1850.988 6250.8290.8310.8300.633 3120.5050.5110.5080.311 1560.3650.3720.3690.172 780.2850.2890.2870.090 00.1950.1990.197 | |||||||
货期：3-5 working days
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