| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | PGKB; PGKPS; dJ417L20.2 |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PGK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PGK2抗体的3篇代表性文献的简要总结(注：内容为模拟示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Development of a specific antibody for human PGK2 and its expression analysis in testicular tissues"*
**作者**：Lee S, et al.
**摘要**：本研究开发了一种针对人PGK2蛋白的特异性多克隆抗体，并通过免疫组化验证其在睾丸组织中的表达定位，证实PGK2在精子发生过程中的重要作用。
2. **文献名称**：*"PGK2 as a metabolic regulator in cancer: Insights from antibody-based detection"*
**作者**：Zhang Y, et al.
**摘要**：通过使用商业化PGK2抗体(货号AB123)，研究揭示了PGK2在肿瘤细胞糖酵解中的调控功能，并发现其高表达与结直肠癌患者预后不良相关。
3. **文献名称**：*"Monoclonal antibody targeting PGK2 inhibits tumor growth in xenograft models"*
**作者**：Chen X, et al.
**摘要**：该团队开发了一种新型PGK2单克隆抗体，体外和体内实验表明其可通过阻断PGK2的酶活性抑制肿瘤能量代谢，为癌症治疗提供潜在策略。
(提示：实际应用中建议通过PubMed或Google Scholar以“PGK2 antibody”为关键词检索最新文献，并筛选涉及抗体开发、验证或应用的论文。)
The PGK2 (phosphoglycerate kinase 2) antibody is a research tool designed to detect and study the PGK2 protein, a key enzyme in the glycolytic pathway. PGK2 catalyzes the conversion of 1.3-bisphosphoglycerate to 3-phosphoglycerate, generating ATP during glycolysis. Unlike its isoform PGK1. which is ubiquitously expressed, PGK2 is primarily testis-specific and plays a critical role in spermatogenesis. However, PGK2 has also been implicated in tumor metabolism, where its re-expression in cancers may support aerobic glycolysis (the Warburg effect) under hypoxic conditions.
PGK2 antibodies are typically developed using recombinant PGK2 protein or peptide immunogens, yielding polyclonal or monoclonal variants. These antibodies are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess PGK2 expression patterns in tissues or cell lines. Specificity is confirmed via knockout controls or siRNA silencing.
Research involving PGK2 antibodies focuses on reproductive biology (e.g., male infertility), cancer metabolism (e.g., tumor progression biomarkers), and metabolic disorders. Its testis-specific expression and potential oncogenic role make it a target for diagnostic or therapeutic exploration. Commercial PGK2 antibodies often include validation data to ensure reliability across experimental models.
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