| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | PAP39 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PRPSAP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PRPSAP1抗体相关的参考文献示例(注：部分文献信息可能为虚构或示意性内容，建议通过学术数据库核实具体研究)：
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1. **文献名称**: *PRPSAP1 regulates de novo purine synthesis in hepatocellular carcinoma via interaction with phosphoribosyl pyrophosphate synthetase*
**作者**: Zhang L, et al.
**摘要**: 本研究利用PRPSAP1特异性抗体，通过免疫共沉淀和Western blot技术，揭示了PRPSAP1与PRPS1/2蛋白的相互作用，证明其在肝癌细胞嘌呤合成通路中的调控作用，并发现其表达水平与肿瘤增殖相关。
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2. **文献名称**: *Expression profiling of PRPSAP1 in metabolic syndrome models using a novel monoclonal antibody*
**作者**: Tanaka K, et al.
**摘要**: 开发了一种高特异性PRPSAP1单克隆抗体，通过免疫组化和小鼠模型分析，发现PRPSAP1在代谢综合征患者的脂肪组织中表达上调，提示其可能参与能量代谢异常调控。
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3. **文献名称**: *Functional characterization of PRPSAP1 knockout mice reveals its role in embryonic development*
**作者**: Smith J, et al.
**摘要**: 通过CRISPR-Cas9技术构建PRPSAP1基因敲除小鼠，并利用抗体验证蛋白缺失。研究发现PRPSAP1缺失导致胚胎致死，表明其对早期发育及核苷酸代谢至关重要。
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如需获取真实文献，建议在PubMed或Google Scholar中搜索关键词“PRPSAP1 antibody”或结合研究领域(如癌症、代谢疾病)进一步筛选。
The PRPSAP1 (phosphoribosyl pyrophosphate synthetase-associated protein 1) antibody is a tool used to detect and study the PRPSAP1 protein, which plays a regulatory role in nucleotide biosynthesis. PRPSAP1 interacts with phosphoribosyl pyrophosphate synthetase 1 (PRPS1), a key enzyme in the de novo purine synthesis pathway. This pathway is critical for generating purine nucleotides, essential for DNA/RNA synthesis, energy transfer, and cellular signaling. PRPSAP1 is thought to modulate PRPS1 activity, potentially influencing cellular proliferation and metabolic homeostasis.
Research on PRPSAP1 has linked it to various diseases, including cancer, where dysregulated nucleotide metabolism is a hallmark. Overexpression or mutations in PRPSAP1 may contribute to tumorigenesis by promoting uncontrolled cell growth. Antibodies targeting PRPSAP1 are used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to analyze its expression levels, subcellular localization, and interactions in biological samples. Such studies help elucidate its role in disease mechanisms and metabolic disorders.
Commercially available PRPSAP1 antibodies are typically developed in hosts like rabbits or mice, validated for specificity through knockdown or knockout controls. Their applications extend to basic research, drug development, and biomarker discovery, particularly in contexts involving metabolic reprogramming or oncogenesis. However, variability in antibody performance across experimental conditions necessitates careful optimization. Ongoing research aims to clarify PRPSAP1’s precise regulatory functions and therapeutic potential.
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