| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | TYRRS; CGI-04; MLASA2; MT-TYRRS |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human YARS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于YARS2抗体的3篇参考文献(信息基于已发表研究综合整理，非真实文献，供参考)：
1. **文献名称**：*YARS2 mutations cause mitochondrial myopathy with lactic acidosis and sideroblastic anemia (MLASA)*
**作者**：Riley LG, et al.
**摘要**：该研究首次报道YARS2基因突变导致线粒体肌病伴乳酸性酸中毒和铁粒幼细胞性贫血(MLASA)，并通过YARS2抗体检测患者肌肉组织中酪氨酰-tRNA合成酶表达水平下降，证实其功能缺陷与线粒体翻译障碍相关。
2. **文献名称**：*Structural and functional analysis of mitochondrial tyrosyl-tRNA synthetase YARS2*
**作者**：Bonnefond L, et al.
**摘要**：通过X射线晶体学解析YARS2蛋白结构，并利用特异性抗体验证其在HEK293细胞线粒体中的定位，揭示YARS2催化结构域的关键氨基酸残基对tRNA结合和酶活性的调控机制。
3. **文献名称**：*A novel YARS2 variant associated with cardiomyopathy and the role of antibody-based diagnostic screening*
**作者**：Almalki A, et al.
**摘要**：报道一例YARS2新发突变导致的扩张型心肌病病例，采用YARS2抗体进行免疫印迹和免疫荧光分析，显示突变蛋白稳定性降低，提示其与线粒体能量代谢异常引发心肌病变相关。
**注**：以上为模拟文献案例，实际文献需通过PubMed或Google Scholar以关键词“YARS2 antibody”或“YARS2 mitochondrial”检索。
The YARS2 antibody targets the mitochondrial tyrosyl-tRNA synthetase (YARS2), an enzyme critical for mitochondrial protein synthesis. YARS2 belongs to the family of aminoacyl-tRNA synthetases (aaRSs), which catalyze the attachment of specific amino acids to their cognate tRNAs during translation. As a mitochondrial matrix protein, YARS2 charges mitochondrial tRNA-Tyr with tyrosine, a step essential for the fidelity of mitochondrial mRNA translation. Mutations in the YARS2 gene are linked to mitochondrial disorders, notably combined oxidative phosphorylation deficiency and myopathy, lactic acidosis, and sideroblastic anemia (MLASA syndrome). These conditions highlight YARS2's role in maintaining mitochondrial function and energy production.
YARS2 antibodies are primarily used in research to study the enzyme's expression, localization, and dysfunction in disease models. They enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding investigations into mitochondrial pathologies. Studies using these antibodies have revealed how YARS2 deficiency disrupts respiratory chain complexes, leading to cellular energy deficits. Additionally, YARS2 antibodies contribute to exploring genotype-phenotype correlations in mitochondrial disorders and potential therapeutic strategies. Their development underscores the growing focus on mitochondrial aaRSs as both biomarkers and targets for treating metabolic diseases.
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