| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | SFTPB; SFTP3; Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe) |
| Entrez GeneID | 6439; |
| WB Predicted band size | 42kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from the Internal region of human SP-B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3条关于NPPB抗体的参考文献摘要示例(基于公开研究内容概括，非真实文献)：
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1. **文献名称**: *Development of a High-Sensitivity Immunoassay for Human B-Type Natriuretic Peptide Using Monoclonal Antibodies*
**作者**: Smith A, et al.
**摘要**: 该研究开发了一种基于单克隆抗体的高灵敏度免疫分析法，用于检测血清中的NPPB(B型钠尿肽前体)，验证了其在心力衰竭早期诊断中的应用潜力，并展示了抗体的高特异性和低交叉反应性。
2. **文献名称**: *NPPB as a Biomarker in Cardiac Hypertrophy: Role of Antibody-Based Detection in Murine Models*
**作者**: Nakagawa Y, et al.
**摘要**: 通过使用抗NPPB的多克隆抗体，本研究在小鼠心脏肥厚模型中揭示了NPPB表达水平与心肌纤维化的相关性，证实了其在病理机制研究中的工具价值。
3. **文献名称**: *Comparative Evaluation of Commercial NPPB Antibodies for Clinical Diagnostics*
**作者**: Johnson R, et al.
**摘要**: 对比分析了多种市售NPPB抗体的灵敏度和特异性，发现某些抗体在ELISA和免疫组化中的性能差异显著，为临床实验室选择合适试剂提供了依据。
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(注：以上为模拟内容，实际文献需通过PubMed、Web of Science等数据库查询。)
Natriuretic peptide precursor B (NPPB) antibody is a specialized tool used in biomedical research to detect and analyze the expression of B-type natriuretic peptide (BNP), a hormone encoded by the NPPB gene. BNP is primarily synthesized and secreted by cardiac ventricular myocytes in response to myocardial stretching and volume overload. It plays a critical role in regulating blood pressure, fluid balance, and cardiovascular homeostasis by promoting vasodilation, natriuresis, and diuresis. Elevated BNP levels are clinically associated with heart failure, myocardial infarction, and other cardiac pathologies, making it a key biomarker for diagnosing and monitoring these conditions.
The NPPB antibody is widely employed in techniques such as immunohistochemistry (IHC), Western blotting, and enzyme-linked immunosorbent assays (ELISA) to localize and quantify BNP in tissue samples or biological fluids. Researchers use this antibody to study the molecular mechanisms underlying cardiovascular diseases, cardiac remodeling, and hypertrophic responses. It is also utilized in preclinical studies to evaluate therapeutic interventions targeting BNP pathways. Commercial NPPB antibodies are typically raised in host species like rabbits or mice using synthetic peptides or recombinant proteins as immunogens. Validation steps, including knockout controls and cross-reactivity assessments, ensure specificity. Despite its utility, variations in antibody performance across experimental conditions necessitate careful optimization. Overall, the NPPB antibody serves as a vital reagent in both basic research and clinical diagnostics, advancing our understanding of cardiovascular health and disease.
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