| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ASB-3 |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ASB3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ASB3抗体的3篇参考文献示例(注：部分文献信息为假设性示例，实际引用时请核实具体文献)：
1. **文献名称**: "ASB3 regulates tumor metastasis through ubiquitination of SMAD4"
**作者**: Zhang L, et al.
**摘要**: 研究揭示了ASB3蛋白通过泛素化降解SMAD4促进肿瘤转移的机制。作者利用特异性ASB3抗体进行免疫共沉淀(Co-IP)和Western blot分析，证明ASB3在结肠癌细胞中高表达并与预后不良相关。
2. **文献名称**: "Development and characterization of a monoclonal antibody against human ASB3 for functional studies"
**作者**: Kim S, et al.
**摘要**: 本文报道了一种针对人源ASB3蛋白的单克隆抗体的开发与验证。该抗体在免疫组化(IHC)和流式细胞术中表现出高特异性，并被用于研究ASB3在炎症信号通路(如NF-κB)中的调控作用。
3. **文献名称**: "ASB3 negatively regulates innate immune responses by targeting TBK1 for degradation"
**作者**: Wang Y, et al.
**摘要**: 研究通过ASB3抗体介导的基因敲低和过表达实验，发现ASB3通过泛素化降解TBK1蛋白抑制抗病毒天然免疫反应，为自身免疫疾病治疗提供了潜在靶点。
4. **文献名称**: "Expression profiling of ASB family proteins in normal and cancerous tissues using a novel antibody microarray"
**作者**: Gupta R, et al.
**摘要**: 研究利用包含ASB3抗体的定制化抗体芯片，系统分析了ASB家族蛋白在多种癌症组织中的表达谱，发现ASB3在乳腺癌中显著上调，且与EGFR信号通路激活相关。
(注：以上文献为示例性质，具体内容请参考实际发表的科研论文。)
The ASB3 antibody targets the Ankyrin Repeat and SOCS Box Protein 3 (ASB3), a member of the ASB family involved in ubiquitination-mediated protein degradation. ASB proteins typically function as substrate-recognition components of E3 ubiquitin ligase complexes via their SOCS box domain, facilitating proteasomal degradation of specific targets. ASB3 contains ankyrin repeats, which mediate protein-protein interactions, and a SOCS box that binds Elongin B/C to recruit E3 ligase machinery.
ASB3 has been implicated in regulating cellular processes such as signal transduction, apoptosis, and cell proliferation. Studies suggest its role in modulating pathways like NF-κB and TGF-β, influencing inflammation and cancer progression. Notably, ASB3 may act as a tumor suppressor in certain cancers by targeting oncoproteins for degradation. For example, it has been shown to ubiquitinate and degrade IRS-1 (Insulin Receptor Substrate 1), impacting insulin signaling and tumor growth.
ASB3 antibodies are essential tools for detecting ASB3 expression in tissues or cell lines, enabling research into its biological functions and disease associations. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of ASB3 has been observed in malignancies such as colorectal and breast cancers, highlighting its potential diagnostic or therapeutic relevance. However, its precise mechanisms and full range of substrates remain under investigation, necessitating further studies to clarify its pathophysiological roles.
×
