| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SPGF7; CATSPER |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CATSPER1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与CATSPER1抗体相关的研究文献概览：
1. **《CATSPER1 required for evoked Ca²⁺ entry and control of flagellar function in sperm》**
- 作者：Ren et al.
- 摘要：首次发现CATSPER1基因编码的精子特异性钙通道对精子活力及受精能力的关键作用，研究中使用特异性抗体定位蛋白在精子鞭毛主段的表达。
2. **《A sperm-specific ion channel critical for sperm motility and male fertility》**
- 作者：Qi et al.
- 摘要：通过基因敲除和抗体标记实验，证实CATSPER1缺失导致精子钙信号异常及雄性不育，抗体用于Western blot和免疫荧光验证蛋白表达缺失。
3. **《Human male infertility caused by mutations in the CATSPER1 channel subunit》**
- 作者：Avenarius et al.
- 摘要：发现人类CATSPER1基因突变与不育症关联，利用抗体进行精子蛋白检测，显示突变患者精子中CATSPER1蛋白表达显著降低。
4. **《CATSPER channel-mediated calcium signaling in male reproduction: An emerging target for infertility》**
- 作者：Smith & Cherutz
- 摘要：综述CATSPER1在生殖中的分子机制，强调其抗体在研究精子钙通道功能及开发不育症诊断工具中的应用潜力。
这些文献聚焦于CATSPER1抗体的功能验证、疾病关联及技术应用，涵盖基础研究到临床转化。
The CATSPER1 antibody is a specialized tool used to detect and study the CATSPER1 protein, a key component of the CatSper (Cation Channel of Sperm) ion channel family. CatSper channels are sperm-specific, pH-sensitive calcium channels essential for sperm hyperactivation, motility, and fertilization. Among the four CatSper subunits (CatSper1-4), CATSPER1 forms the pore-forming subunit and is critical for channel assembly and function. Mutations in the CATSPER1 gene are linked to male infertility due to impaired sperm motility and failed egg penetration.
CATSPER1 antibodies are primarily employed in reproductive biology research to investigate sperm physiology, infertility mechanisms, and contraceptive development. These antibodies enable the localization and quantification of CATSPER1 in sperm cells using techniques like Western blotting, immunofluorescence, and flow cytometry. Their specificity helps differentiate CATSPER1 from other ion channels and assess its expression levels in pathological conditions.
Recent studies also explore CATSPER1's role beyond fertility, including potential signaling functions in cancer cells. However, challenges persist in antibody validation due to the channel's structural complexity and low abundance in sperm membranes. Despite this, CATSPER1 antibodies remain vital for understanding calcium signaling in sperm and developing targeted therapies for infertility or non-hormonal contraceptives.
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