| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CATSPER4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CATSPER4抗体的3篇参考文献的简要概括：
1. **文献名称**：*CATSPER4 is a novel regulator of sperm calcium channel activity in humans*
**作者**：Smith AB, et al.
**摘要**：研究利用特异性CATSPER4抗体，通过免疫印迹和免疫荧光技术，证实了CATSPER4蛋白在人类精子鞭毛中的定位，并发现其功能缺陷与男性不育相关。抗体被用于检测蛋白表达水平及亚细胞分布。
2. **文献名称**：*Expression and functional analysis of CATSPER family proteins in mouse testis*
**作者**：Tanaka Y, et al.
**摘要**：通过构建CATSPER4多克隆抗体，研究者在小鼠睾丸组织中发现CATSPER4与其他CATSPER家族成员(如CATSPER1/2)共定位，并揭示了其在精子钙离子内流中的协同作用。抗体用于免疫组化验证蛋白表达模式。
3. **文献名称**：*Antibody-based characterization of CATSPER4 in sperm motility disorders*
**作者**：Wang C, et al.
**摘要**：研究采用CATSPER4单克隆抗体对不育患者精子样本进行流式细胞术分析，发现CATSPER4表达降低与精子运动能力受损显著相关，提示其可作为男性不育的潜在生物标志物。
注：以上文献为示例性质，实际引用时需核实具体来源及内容准确性。若需获取真实文献，建议通过PubMed或Web of Science以关键词“CATSPER4 antibody”检索近期研究。
The CatSper4 antibody is a research tool designed to detect the CatSper4 protein, a key subunit of the CatSper (Cation Channel of Sperm) family. CatSper channels are sperm-specific, pH-dependent, voltage-gated calcium channels essential for sperm hyperactivation, motility, and fertilization. Among the four pore-forming subunits (CatSper1-4), CatSper4 plays a critical role in forming the functional channel complex, particularly in mediating calcium influx during sperm capacitation and the acrosome reaction. Dysregulation of CatSper4 has been linked to male infertility, making it a target for studying reproductive disorders.
Antibodies against CatSper4 are widely used in reproductive biology to investigate its expression, localization, and function in sperm cells. They enable techniques like Western blotting, immunofluorescence, and immunohistochemistry to study protein distribution in sperm flagella and assess changes under pathological or experimental conditions. These antibodies also aid in exploring CatSper4's interaction with other channel subunits or regulatory proteins, providing insights into channel assembly and signaling mechanisms. Recent studies have utilized CatSper4 antibodies to evaluate its potential as a diagnostic marker or therapeutic target for infertility. However, challenges remain in ensuring antibody specificity due to structural similarities among CatSper subunits. Validation via knockout models or peptide competition is often recommended for accurate experimental interpretation.
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