| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NAR; NEB1; CPSF30 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CPSF4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与CPSF4抗体相关的模拟参考文献(基于研究领域常见主题构建，非真实文献)：
1. **标题**：*CPSF4 mediates viral RNA binding to promote Zika virus replication*
**作者**：Chen X, et al.
**摘要**：利用CPSF4特异性抗体进行免疫共沉淀实验，揭示CPSF4通过结合寨卡病毒RNA的3'非编码区，调控病毒颗粒的组装与释放。
2. **标题**：*CPSF4 overexpression correlates with poor prognosis in glioblastoma*
**作者**：Kim H, et al.
**摘要**：通过CPSF4抗体进行免疫组化分析，证实CPSF4在胶质母细胞瘤组织中异常高表达，其水平与患者生存率负相关，并促进肿瘤细胞增殖。
3. **标题**：*Development of a monoclonal antibody against CPSF4 for RNA processing studies*
**作者**：Li R, et al.
**摘要**：报道一种新型兔源CPSF4单克隆抗体的开发与验证，应用于Western blot和免疫荧光，证实CPSF4在细胞核内的动态定位及其与mRNA加工复合体的相互作用。
4. **标题**：*CPSF4 inhibition suppresses HIV-1 replication by impairing viral mRNA polyadenylation*
**作者**：Gupta S, et al.
**摘要**：使用CPSF4抗体进行功能阻断实验，证明抑制CPSF4会干扰HIV-1 mRNA的多聚腺苷酸化过程，从而降低病毒蛋白表达水平。
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**注**：以上文献为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索确认。建议结合具体研究目的筛选关键词(如“CPSF4 antibody application”或“CPSF4 immunological assays”)进行精准查询。
The CPSF4 antibody targets the Cleavage and Polyadenylation Specificity Factor subunit 4 (CPSF4), a critical component of the CPSF complex involved in mRNA 3′-end processing. CPSF4. also known as CPSF30. plays a key role in the cleavage and polyadenylation of pre-mRNAs, a fundamental step in eukaryotic gene expression. It recognizes the canonical polyadenylation signal (AAUAAA) in nascent RNA transcripts and recruits other processing factors to ensure accurate termination and poly-A tail addition, which influences mRNA stability, nuclear export, and translation.
CPSF4 antibodies are widely used in research to study transcriptional regulation, particularly in contexts like viral replication (e.g., HIV), cancer, and neurodevelopmental disorders. Studies have linked CPSF4 dysregulation to alternative polyadenylation events, which can alter gene expression patterns in tumors or during viral infection. Structurally, CPSF4 contains RNA-binding motifs (RRMs) and a zinc-finger domain, enabling interactions with RNA and protein partners.
Researchers employ CPSF4 antibodies in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, localization, and functional interactions. These tools help elucidate how CPSF4 contributes to cellular processes and disease mechanisms, making it a potential therapeutic or diagnostic target.
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