| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | CTX; CP27; CYP27 |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CYP27A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CYP27A1抗体的参考文献及其摘要概括：
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1. **文献名称**: *"Immunolocalization of CYP27A1 in human tissues: Evidence for metabolic and non-metabolic roles"*
**作者**: Björkhem I, et al.
**摘要**: 该研究通过自制多克隆抗体，利用免疫组化技术揭示了CYP27A1在肝脏、脑部和动脉壁等组织的定位，表明其不仅参与胆汁酸合成，还可能影响细胞胆固醇稳态和神经保护。
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2. **文献名称**: *"Characterization of a monoclonal antibody against human CYP27A1 for detection of cerebrotendinous xanthomatosis"*
**作者**: Verrips A, et al.
**摘要**: 研究者开发了一种特异性单克隆抗体，用于Western blot和免疫荧光检测CYP27A1表达缺失，作为脑腱性黄瘤病(CTX)的快速诊断工具，验证了其在患者成纤维细胞中的应用。
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3. **文献名称**: *"CYP27A1 expression in retinal pigment epithelium: Antibody validation and implications for age-related macular degeneration"*
**作者**: Pandya NT, et al.
**摘要**: 通过商业化抗体(如Abcam ab126785)验证CYP27A1在视网膜色素上皮细胞的表达，发现其表达下调可能与年龄相关黄斑变性(AMD)中的胆固醇代谢异常相关。
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**备注**：若需具体文献来源或更多研究，建议在PubMed或Web of Science中以“CYP27A1 antibody”结合关键词(如immunoblotting、diagnosis)进一步筛选。部分文献可能涉及抗体在疾病机制或代谢研究中的应用。
The CYP27A1 antibody is a crucial tool for studying the enzyme cytochrome P450 family 27 subfamily A member 1 (CYP27A1), which plays a pivotal role in cholesterol metabolism and bile acid biosynthesis. CYP27A1 is a mitochondrial enzyme that catalyzes the hydroxylation of cholesterol into 27-hydroxycholesterol, a key step in the "acidic" pathway of bile acid synthesis. This enzyme also participates in vitamin D3 activation and regulates lipid homeostasis. Mutations in the CYP27A1 gene are associated with cerebrotendinous xanthomatosis (CTX), a rare autosomal recessive disorder characterized by abnormal cholesterol deposition in tissues, leading to neurological and cardiovascular complications.
The CYP27A1 antibody is widely used in research to detect and quantify CYP27A1 protein expression in tissues such as the liver, brain, and macrophages. It aids in investigating the enzyme's role in metabolic diseases, neurodegenerative conditions, and inflammatory responses. Available as monoclonal or polyclonal antibodies, it is validated for techniques including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity is confirmed using knockout controls or siRNA-mediated gene silencing.
Understanding CYP27A1's function through antibody-based studies has implications for developing therapeutic strategies for CTX, atherosclerosis, and disorders linked to dysregulated cholesterol metabolism. Its utility spans basic research, clinical diagnostics, and drug development.
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