| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CYP11A; CYPXIA1; P450SCC |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CYP11A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于 **CYP11A1抗体** 的参考文献示例(内容为模拟，仅供参考)：
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1. **文献名称**: *"Immunolocalization of CYP11A1 in human adrenal glands and gonads"*
**作者**: Hanukoglu I. et al.
**摘要**: 使用特异性CYP11A1抗体研究其在人类肾上腺和性腺组织中的定位，证实其在类固醇激素合成细胞线粒体中的高表达，为研究先天性肾上腺增生症提供依据。
2. **文献名称**: *"CYP11A1 expression in placental trophoblasts: A role in progesterone synthesis"*
**作者**: Tuckey R.C. et al.
**摘要**: 通过Western blot和免疫组化技术，验证CYP11A1抗体在胎盘滋养层细胞中的特异性，揭示其在孕酮合成中的关键作用及与妊娠并发症的潜在关联。
3. **文献名称**: *"Species-specific cross-reactivity of CYP11A1 antibodies: Implications for comparative studies"*
**作者**: Payne A.H. et al.
**摘要**: 评估不同物种(小鼠、大鼠、人类)中CYP11A1抗体的交叉反应性，为跨物种类固醇生成研究提供抗体选择指导，强调抗体验证的重要性。
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*注：以上文献为示例，实际引用时需核实真实文献信息。建议通过PubMed或Google Scholar以“CYP11A1 antibody”为关键词检索最新研究。*
CYP11A1. also known as cholesterol side-chain cleavage enzyme (P450scc), is a mitochondrial cytochrome P450 enzyme critical for steroid hormone biosynthesis. It catalyzes the first and rate-limiting step in steroidogenesis, converting cholesterol to pregnenolone by cleaving its side chain. This reaction occurs in steroidogenic tissues, including the adrenal glands, gonads, and placenta, and is essential for producing glucocorticoids, mineralocorticoids, and sex hormones.
CYP11A1 antibodies are immunological tools used to detect and study the expression, localization, and regulation of this enzyme in research and diagnostic contexts. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptide sequences or recombinant protein fragments. They are widely applied in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess CYP11A1 levels in tissue samples, cell cultures, or animal models.
Research involving CYP11A1 antibodies often focuses on disorders linked to steroidogenesis dysregulation, such as congenital adrenal hyperplasia, polycystic ovary syndrome (PCOS), and hormone-dependent cancers. Additionally, they help explore the enzyme's role in developmental biology, reproductive health, and stress responses. Reliable CYP11A1 antibodies are critical for validating gene-editing models (e.g., CRISPR/Cas9) and screening for therapeutic targets in endocrine pathologies. Proper validation, including knockout controls and tissue-specific staining patterns, ensures antibody specificity and accuracy in experimental outcomes.
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