| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CPT7; CYP17; S17AH; P450C17 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CYP17A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP17A1抗体的3篇参考文献示例(内容为模拟概括，仅供参考)：
1. **文献名称**：*"Immunohistochemical localization of CYP17A1 in bovine adrenal glands and testes using a novel monoclonal antibody"*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种针对CYP17A1的单克隆抗体，并通过免疫组化验证其在牛肾上腺和睾丸组织中的特异性定位，证实了其在类固醇生成细胞中的高表达。
2. **文献名称**：*"Validation of a CYP17A1 antibody for Western blot analysis in human adrenal hyperplasia studies"*
**作者**：Brown C, et al.
**摘要**：文章验证了一种兔多克隆CYP17A1抗体的特异性，通过Western blot和免疫荧光分析，发现其在先天性肾上腺增生症患者组织中表达异常，为疾病机制研究提供工具。
3. **文献名称**：*"CYP17A1 expression in prostate cancer: A comparative study using commercial antibodies"*
**作者**：Johnson R, et al.
**摘要**：比较了四种市售CYP17A1抗体在前列腺癌组织中的性能，筛选出高特异性抗体，并揭示CYP17A1在雄激素合成通路中的潜在治疗靶点作用。
(注：以上文献为虚构示例，实际引用需查询PubMed、Google Scholar等数据库获取真实文献。)
The CYP17A1 antibody is a crucial tool in studying the cytochrome P450 17A1 enzyme, a key player in steroid hormone biosynthesis. CYP17A1. located in the endoplasmic reticulum, exhibits dual enzymatic activities: 17α-hydroxylase and 17.20-lyase. These functions enable it to catalyze the conversion of pregnenolone and progesterone to 17α-hydroxypregnenolone and 17α-hydroxyprogesterone (precursors for glucocorticoids and androgens) and subsequently to dehydroepiandrosterone (DHEA) and androstenedione, respectively. This enzyme is primarily expressed in adrenal glands, gonads, and placenta, making it vital for cortisol, estrogen, and androgen production.
Antibodies targeting CYP17A1 are widely used in research and diagnostics to localize its expression in tissues, quantify protein levels via techniques like immunohistochemistry, Western blotting, or ELISA, and investigate disorders linked to its dysfunction. For instance, CYP17A1 deficiency causes congenital adrenal hyperplasia (17α-hydroxylase deficiency), leading to hypertension and sexual development abnormalities. Conversely, its overexpression in certain cancers (e.g., prostate, adrenal tumors) has spurred interest in therapeutic targeting. Drugs like abiraterone, a CYP17A1 inhibitor, are used to treat advanced prostate cancer by suppressing androgen synthesis.
These antibodies also aid in elucidating regulatory mechanisms of steroidogenesis and evaluating pharmacological interventions, bridging molecular insights with clinical applications.
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