| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | DBP5; RNAh; DDX19 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DDX19B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DDX19B抗体的3篇假设性文献示例(内容基于领域知识构建，非真实文献)：
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1. **文献名称**：*DDX19B regulates mRNA export and innate immune signaling in antiviral responses*
**作者**：Schmidt, E. et al.
**摘要**：本研究通过DDX19B特异性抗体进行免疫共沉淀实验，发现DDX19B与细胞质mRNA输出复合物相互作用，并在病毒RNA刺激下激活干扰素信号通路，揭示其通过调控mRNA核质转运参与先天免疫应答的机制。
2. **文献名称**：*Antibody-based profiling of DDX19B reveals its role in HCV replication*
**作者**：Tanaka, K. et al.
**摘要**：利用DDX19B单克隆抗体进行亚细胞定位分析，发现丙肝病毒(HCV)感染导致DDX19B从核孔重新分布至病毒复制复合体，并通过基因敲低实验证明DDX19B对HCV RNA复制具有关键调控作用。
3. **文献名称**：*DDX19B antibody screening identifies its overexpression in colorectal cancer metastasis*
**作者**：Liu, X. et al.
**摘要**：通过组织芯片结合DDX19B抗体免疫组化分析，发现DDX19B在结直肠癌转移灶中显著高表达，体外实验证实其通过促进EMT(上皮间质转化)增强癌细胞侵袭能力，提示其作为转移标志物的潜力。
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*注：以上文献为示例性质，实际研究需参考真实发表的论文。*
**Background of DDX19B Antibody**
DDX19B (DEAD-box helicase 19B) is a member of the DEAD-box RNA helicase family, characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs and ATP-dependent RNA helicase activity. It plays critical roles in mRNA metabolism, including nuclear export, translation termination, and stress granule dynamics. DDX19B localizes near nuclear pore complexes, where it facilitates mRNA export by remodeling ribonucleoprotein particles and interacting with export machinery like NXF1. It also participates in resolving mRNA secondary structures, ensuring efficient cytoplasmic translation.
Antibodies targeting DDX19B are essential tools for studying its expression, localization, and functional interactions. These antibodies are commonly validated in applications such as Western blotting, immunoprecipitation, and immunofluorescence. Researchers use DDX19B-specific antibodies to explore its involvement in cellular stress responses, viral replication (e.g., HIV-1), and diseases linked to RNA dysregulation, such as neurodegeneration or cancer. Commercial DDX19B antibodies are typically raised against epitopes within its conserved helicase domains, ensuring specificity across species like humans, mice, and rats. Recent studies also highlight DDX19B's potential as a therapeutic target, driving demand for high-quality antibodies to dissect its mechanistic roles in RNA biology and disease pathology.
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